TY - JOUR
T1 - Anti-contamination device for cryogenic soft X-ray diffraction microscopy
AU - Huang, Xiaojing
AU - Miao, Huijie
AU - Nelson, Johanna
AU - Turner, Joshua
AU - Steinbrener, Jan
AU - Shapiro, David
AU - Kirz, Janos
AU - Jacobsen, Chris
N1 - Funding Information:
We thank the Division of Materials Sciences and Engineering, Office of Basic Energy Sciences, at the Department of Energy for support of X-ray diffraction microscopy methods and instrumentation development under contract DE-FG02-07ER46128 . We also thank the National Institute for General Medical Services at the National Institutes for Health for support of the application of this method to biological imaging under contract 5R21EB6134. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Finally, we thank Stefano Marchesini and George Meigs of ALS for the fruitful discussions related to this project, and Richard Celestre and Tony Warwick of ALS for help and advice.
PY - 2011/5/11
Y1 - 2011/5/11
N2 - Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens.
AB - Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens.
KW - Anti-contamination
KW - Cryogenic X-ray microscopy
KW - Diffraction microscopy
KW - X-ray Imaging
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U2 - 10.1016/j.nima.2011.02.085
DO - 10.1016/j.nima.2011.02.085
M3 - Article
C2 - 21547016
AN - SCOPUS:79953317209
SN - 0168-9002
VL - 638
SP - 171
EP - 175
JO - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
JF - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
IS - 1
ER -