Anti-phospholipid antibodies are elevated and functionally active in chronic rhinosinusitis with nasal polyps

Jacob G. Eide, Jeffanie Wu, Whitney W. Stevens, Junqin Bai, Songwang Hou, Julia H. Huang, Jacob Rosenberg, Paul Utz, Stephanie Shintani-Smith, David B. Conley, Kevin C. Welch, Robert C. Kern, Kathryn E. Hulse, Anju T. Peters, Leslie C. Grammer, Ming Zhao, Paul Lindholm, Robert P. Schleimer, Bruce K. Tan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Background: Polyps from patients with chronic rhinosinusitis with nasal polyps (CRSwNP) contain increased levels of autoreactive antibodies, B cells and fibrin deposition. Anti-phospholipid antibodies (APA) are autoantibodies known to cause thrombosis but have not been implicated in chronic rhinosinusitis (CRS). Objective: To compare APA levels (anti-cardiolipin, anti-phosphatidylethanolamine (anti-PE), and anti-β2-glycoprotein (anti-B2GP)) in nasal polyp (NP) tissue with tissue from control and CRS without nasal polyp (CRSsNP) patients, we tested whether NP antibodies affect coagulation, and correlate APAs with anti-dsDNA IgG and markers of coagulation. Methods: Patient specimens were assayed for APA IgG, anti-dsDNA IgG and thrombin-anti-thrombin (TaT) complex by ELISA. Antibodies from a subset of specimens were tested for modified activated partial thromboplastin time (aPTT) measured on an optical-mechanical coagulometer. Results: Anti-cardiolipin IgG in NP was 5-fold higher than control tissue (p <.0001). NP antibodies prolonged aPTT compared to control tissue antibodies at 400 µg/mL (36.7 s vs. 33.8 s, p =.024) and 600 µg/mL (40.9 s vs. 34.7 s, p =.0037). Anti-PE IgG antibodies were increased in NP (p =.027), but anti-B2GP IgG was not significantly higher (p =.084). All APAs correlated with anti-dsDNA IgG levels, which were also elevated (R =.77,.71 and.54, respectively, for anti-cardiolipin, anti-PE, and anti-B2GP; all p <.001), but only anti-cardiolipin (R =.50, p =.0185) and anti-PE (R = 0.45, p =.037) correlated with TaT complex levels. Conclusions: APA IgG antibodies are increased in NP and correlate with autoreactive tissue antibodies. NP antibodies have in vitro anti-coagulant activity similar to those observed in anti-phospholipid syndrome, suggesting that they may have pro-coagulant effects in polyp tissue.

Original languageEnglish (US)
Pages (from-to)954-964
Number of pages11
JournalClinical and Experimental Allergy
Issue number8
StatePublished - Aug 2022


  • anti-phospholipid antibodies
  • nasal polyps
  • rhinosinusitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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