Anti-skin aging effect of GX-50

Xue Feng Hou, Xiao Fei Song, Zhao Xia Wang*, Jing Bo Dai, Mao Ping Tang, Xiao Hui Chen, Xiao Jing Song, Ping Gao, Zhong Dong Qiao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Objective: To investigate the protective effect of GX-50 against skin aging. Methods: GX-50 was applied on the back of mice aged 20 months, and the effect of GX-50 on mouse skin was observed with HE staining and immunohistochemistry. Human skin fibroblasts were primarily cultured, senescent cell model was established by H2O2 induction, and blank control group, DMSO group, GX-50 group, H2O2 group, and GX-50+H2O2 group (pretreatment with GX-50+H2O2 treatment) were divided. The anti-aging effect of GX-50 on primary human skin fibroblasts was examined by SA-β-Gal staining, as well as determination of secreted malondialdehyde (MDA) concentration and intracellular reactive oxygen species (ROS) content. The irritation characteristics of GX-50 were detected by Draize eye test. Results: The histological observation revealed that the GX-50 treated skin exhibited better appearance and higher collagen content. β-galactosidase in human skin fibroblasts was reduced by GX-50. Compared with blank control group, the secretion of MDA was down-regulated by 30.45% and the fluorescence value of ROS was down-regulated by 8.03% in GX-50 group (P<0.05). Compared with H2O2 group, the secretion of MDA was down-regulated by 61.46% and the fluorescence value of ROS was down-regulated by 8.36% in GX-50+H2O2 group (P<0.05). Draize eye test showed the irritation score of GX-50 was 0. Conclusion: GX-50 has protective and anti-aging effect on skin, and may serve as a potential cosmetic additive against skin aging.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalJournal of Shanghai Jiaotong University (Medical Science)
Issue number2
StatePublished - Feb 2013


  • Collagen
  • GX-50
  • Malondialdehyde
  • Reactive oxygen species
  • Skin aging

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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