Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

Young Hyo Kim, Chang Shin Park, Dae Hyun Lim, Byong Kwan Son, Jeong Hee Kim, Sung Hye Ahn, Bruce S. Bochner, Kwangmin Na, Tae Young Jang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalAmerican Journal of Rhinology and Allergy
Volume27
Issue number3
DOIs
StatePublished - May 1 2013

Fingerprint

Sialic Acid Binding Immunoglobulin-like Lectins
Anti-Allergic Agents
Inflammation
Ovalbumin
Bronchoalveolar Lavage Fluid
Eosinophils
Antibodies
Interleukin-13
Nasal Cavity
Interleukin-5
Nose
Interleukin-4
Immunoglobulin E
Control Groups
Allergic Rhinitis
Nasal Mucosa
Serum
Mucous Membrane
Neutrophils
Theoretical Models

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Kim, Young Hyo ; Park, Chang Shin ; Lim, Dae Hyun ; Son, Byong Kwan ; Kim, Jeong Hee ; Ahn, Sung Hye ; Bochner, Bruce S. ; Na, Kwangmin ; Jang, Tae Young. / Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis. In: American Journal of Rhinology and Allergy. 2013 ; Vol. 27, No. 3. pp. 187-191.
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title = "Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis",
abstract = "Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.",
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Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis. / Kim, Young Hyo; Park, Chang Shin; Lim, Dae Hyun; Son, Byong Kwan; Kim, Jeong Hee; Ahn, Sung Hye; Bochner, Bruce S.; Na, Kwangmin; Jang, Tae Young.

In: American Journal of Rhinology and Allergy, Vol. 27, No. 3, 01.05.2013, p. 187-191.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

AU - Kim, Young Hyo

AU - Park, Chang Shin

AU - Lim, Dae Hyun

AU - Son, Byong Kwan

AU - Kim, Jeong Hee

AU - Ahn, Sung Hye

AU - Bochner, Bruce S.

AU - Na, Kwangmin

AU - Jang, Tae Young

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

AB - Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

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