Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

Young Hyo Kim, Chang Shin Park, Dae Hyun Lim*, Byong Kwan Son, Jeong Hee Kim, Sung Hye Ahn, Bruce S. Bochner, Kwangmin Na, Tae Young Jang

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalAmerican Journal of Rhinology and Allergy
Volume27
Issue number3
DOIs
StatePublished - May 1 2013

Fingerprint

Sialic Acid Binding Immunoglobulin-like Lectins
Anti-Allergic Agents
Inflammation
Ovalbumin
Bronchoalveolar Lavage Fluid
Eosinophils
Antibodies
Interleukin-13
Nasal Cavity
Interleukin-5
Nose
Interleukin-4
Immunoglobulin E
Control Groups
Allergic Rhinitis
Nasal Mucosa
Serum
Mucous Membrane
Neutrophils
Theoretical Models

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Kim, Young Hyo ; Park, Chang Shin ; Lim, Dae Hyun ; Son, Byong Kwan ; Kim, Jeong Hee ; Ahn, Sung Hye ; Bochner, Bruce S. ; Na, Kwangmin ; Jang, Tae Young. / Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis. In: American Journal of Rhinology and Allergy. 2013 ; Vol. 27, No. 3. pp. 187-191.
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title = "Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis",
abstract = "Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.",
author = "Kim, {Young Hyo} and Park, {Chang Shin} and Lim, {Dae Hyun} and Son, {Byong Kwan} and Kim, {Jeong Hee} and Ahn, {Sung Hye} and Bochner, {Bruce S.} and Kwangmin Na and Jang, {Tae Young}",
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Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis. / Kim, Young Hyo; Park, Chang Shin; Lim, Dae Hyun; Son, Byong Kwan; Kim, Jeong Hee; Ahn, Sung Hye; Bochner, Bruce S.; Na, Kwangmin; Jang, Tae Young.

In: American Journal of Rhinology and Allergy, Vol. 27, No. 3, 01.05.2013, p. 187-191.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

AU - Kim, Young Hyo

AU - Park, Chang Shin

AU - Lim, Dae Hyun

AU - Son, Byong Kwan

AU - Kim, Jeong Hee

AU - Ahn, Sung Hye

AU - Bochner, Bruce S.

AU - Na, Kwangmin

AU - Jang, Tae Young

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

AB - Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

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DO - 10.2500/ajra.2013.27.3866

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