Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

Young Hyo Kim, Chang Shin Park, Dae Hyun Lim*, Byong Kwan Son, Jeong Hee Kim, Sung Hye Ahn, Bruce S. Bochner, Kwangmin Na, Tae Young Jang

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalAmerican Journal of Rhinology and Allergy
Volume27
Issue number3
DOIs
StatePublished - May 1 2013

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

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