Antibodies to lipoprotein lipase application to perfused heart

Michael C. Schotz, Twu Jer-Shung, Mary E. Pedersen, Chen Chi-Hong, Arlene S. Garfinkel, Jayme Borensztajn

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

An antibody was prepared against purified rat heart lipoprotein lipase. 1. 1. This antibody showed marked species specificity. It inhibited almost totally the lipoprotein lipase activity from all rat tissues examined (i.e., heart, adipose, postheparin plasma, and mammary gland), while having no effect on the activity of lipoprotein lipase partially purified from rabbit, guinea pig and bovine heart and from bovine milk. The antibody also had no effect on the hepatic lipase activity of rat postheparin plasma. 2. 2. After antibody to rat heart lipoprotein lipase was recirculated for 5 min through isolated rat hearts, little or no lipoprotein lipase activity could be detected in the perfusate during 0-20 s of a subsequent non-recirculating perfusion with buffer containing 1 unit heparin/ml. 3. 3. Following recirculation of antibody to lipoprotein lipase for 10 min and a non-recirculating perfusion with buffer for 2 min, the hearts no longer oxidized any significant amounts of 14C-labelled palmitate chylomicron triacylglycerol fatty acid to 14CO2 during a 15-min perfusion. The data give compelling evidence that the functional fraction of lipoprotein lipase in hearts is at the endothelial cell surface accessible to lipoprotein lipase antibody.

Original languageEnglish (US)
Pages (from-to)214-224
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume489
Issue number2
DOIs
StatePublished - Nov 24 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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