Anticarcinogenic action of diallyl sulfide in hamster buccal pouch and forestomach

Moolky Nagabhushan, Darren Line, Peter J. Polverini, Dennis B. Solt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The anticarcinogenic action of the garlic constituent diallyl sulfide (DAS), was examined in the hamster buccal pouch and forestomach. Groups of hamsters were topically treated, for up to 14 weeks, with a 0.5% solution of the buccal pouch and forestomach carcinogen 7,12-dimethylbenz[a]anthracene (DMBA). Prior to, during and after DMBA treatment, groups of hamsters were also treated, on alternate days, with a 1% solution of DAS. In addition to tumor formation, the induction of γ-glutamyl transpeptidase (γGT) buccal pouch epithelial lesions served as an additional presumptive index of in vivo carcinogenesis/anticarcinogenesis. DAS resulted in a significant reduction in buccal pouch tumor frequency, buccal pouch tumor burden, buccal pouch γGT lesion frequency and forestomach tumor frequency. In a separate experiment, DAS also reduced the level of autoradiographically quantified unscheduled DNA repair synthesis (UDS) in pieces of hamster buccal pouch concurrently exposed in vitro to the potent buccal pouch carcinogen N-methyl-N-benzylnitrosamine (MBN). This study demonstrates that DAS is an effective anticarcinogenic agent in squamous mucosa of the hamster and suggests novel cost-effective strategies for the rapid identification of tissue-specific anticarcinogens and a quantitative assessment of their efficacy.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalCancer Letters
Volume66
Issue number3
DOIs
StatePublished - Oct 21 1992

Keywords

  • anticarcinogens
  • diallyl sulfide
  • oral carcinogenesis
  • unscheduled DNA synthesis
  • γ-glutamyl transpeptidase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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