The induction of new suppressor T cells (T(s 2)) by suppressive extracts (T(s)F) from L-glutamic acid 50-L-tyrosine 50 (GT) nonresponder mice was examined. Incubation of normal spleen cells with allogeneic GT-T(s)F for 2 days in vitro led to the generation of T(s 2) cells able to suppress subsequent responses to the immunogen GT-methylated bovine serum albumin (GT-MBSA) in vivo. This induction occurred efficiently when T(s)F donor and target cells differed at all of H-2, including the I-J subregion. B10.BR (H-2(k)) GT-T(s)F, absorbed on, then acid eluted from GT-Sepharose and anti-I-J(k) [B10.A (3R) anti-B10.A (5R)]-Sepharose in a sequential fashion could nduce BALB/c (H-2(d)) spleen cells to become T(s 2) only if nanogram quantities of GT were added to the purified GT-T(s)F. This indicates a requirement for a molecule or molecular complex possessing both I-J determinants and antigen (GT)-binding specificity, together with GT itself, for T(s 2) induction. The induced T(s 2) are I-J +, since their function can be eliminated by treatment with anti-I-J(k) plus C. These I-J determinants are coded for by the precursor of the T(s 2) and do not represent passively adsorbed, I-J coded T(s)F, since anti-I-J(k) antiserum [3R x DBA/2)F 1 anti-5R] which cannot recognize the BALB/c (I-J(d)) T(s)F used for induction still eliminates the activity of induced A/J (I-J(k)) T(s 2). These data provide further evidence for and information about the minimum of two T cells involved in antigen-specific suppressor T cell systems.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - Dec 1 1978|
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