Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model

Ying Ying Huang, Anton Wintner, Patrick C. Seed, Timothy Brauns, Jeffrey A. Gelfand*, Michael R. Hamblin

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Drug-resistant urinary tract infections (UTIs) are difficult and sometimes impossible to treat. Many UTIs are caused by uropathogenic Escherichia coli (UPEC). We developed an intact rat model of UTI, by catheterizing female rats and introducing a bioluminescent UPEC strain into the female rat bladder which lasted for up to six days. We recently showed that antimicrobial photodynamic inactivation (aPDI) of a bacterial infection mediated by the well-known phenothiazinium salt, methylene blue (MB) could be strongly potentiated by addition of the non-toxic salt potassium iodide (KI). In the intact rat model we introduced MB into the bladder by catheter, followed by KI solution and delivered intravesicular illumination with a diffusing fiber connected to a 1 W 660 nm laser. Bioluminescent imaging of the bacterial burden was carried out during the procedure and for 6 days afterwards. Light-dose dependent loss of bioluminescence was observed with the combination of MB followed by KI, but recurrence of infection was seen the next day in some cases. aPDT with MB + KI gave a significantly shorter duration of infection compared to untreated controls. aPDT with MB alone was the least effective. No signs of aPDT damage to the bladder lining were detected. This procedure to treat urinary tract infections without antibiotics by using already approved pharmaceutical substances (MB and KI) may have clinical applicability, either initially as a stand-alone therapy, or as an adjunct to antibiotic therapy by a rapid and substantial reduction of the bacterial burden.

Original languageEnglish (US)
Article number7257
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Potassium Iodide
Methylene Blue
Photochemotherapy
Urinary Tract Infections
Uropathogenic Escherichia coli
Urinary Bladder
Salts
Anti-Bacterial Agents
Infection
Lighting
Bacterial Infections
Pharmaceutical Preparations
methylene iodide
Lasers
Catheters
Light
Recurrence
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Huang, Ying Ying ; Wintner, Anton ; Seed, Patrick C. ; Brauns, Timothy ; Gelfand, Jeffrey A. ; Hamblin, Michael R. / Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model. / Huang, Ying Ying; Wintner, Anton; Seed, Patrick C.; Brauns, Timothy; Gelfand, Jeffrey A.; Hamblin, Michael R.

In: Scientific reports, Vol. 8, No. 1, 7257, 01.12.2018.

Research output: Contribution to journalArticle

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