Antiparallel Side-by-Side Heterodimer for Sequence-Specific Recognition in the Minor Groove of DNA by a Distamycin/ 1 -Methylimidazole-2-carboxamide-netropsin Pair

Milan Mrksich, Peter B. Dervan*

*Corresponding author for this work

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

The two peptides distamycin A (D) and 1-methylimidazole-2-carboxamide-netropsin (2-ImN) simultaneously bind the five-base-pair sequence 5′-TGTTA-3′. Footprinting experiments indicate that both molecules are necessary for binding to this sequence while affinity cleaving data define the orientations with which each peptide binds to this sequence. The footprinting and affinity cleaving data are consistent with a model wherein the two peptides bind as a side-by-side antiparallel heterodimer in the minor groove of double-helical DNA.

Original languageEnglish (US)
Pages (from-to)2572-2576
Number of pages5
JournalJournal of the American Chemical Society
Volume115
Issue number7
DOIs
StatePublished - Apr 1 1993

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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