Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin

Stephen Soukasene; Daniel J. Toft; Tyson J. Moyer; Hsuming Lu; Hyung Kun Lee; Stephany M. Standley; Vincent L. Cryns; Samuel I. Stupp

doi:10.1021/nn203343z

Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin

Stephen Soukasene, Daniel J. Toft, Tyson J. Moyer, Hsuming Lu, Hyung Kun Lee, Stephany M. Standley, Vincent L. Cryns^*, Samuel I. Stupp

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

168 Scopus citations

Abstract

Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

Original language	English (US)
Pages (from-to)	9113-9121
Number of pages	9
Journal	ACS nano
Volume	5
Issue number	11
DOIs	https://doi.org/10.1021/nn203343z
State	Published - Nov 22 2011

Keywords

camptothecin
cancer therapy
drug delivery
nanofiber
peptide amphiphile
self-assembly

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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title = "Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin",

abstract = "Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.",

keywords = "camptothecin, cancer therapy, drug delivery, nanofiber, peptide amphiphile, self-assembly",

author = "Stephen Soukasene and Toft, {Daniel J.} and Moyer, {Tyson J.} and Hsuming Lu and Lee, {Hyung Kun} and Standley, {Stephany M.} and Cryns, {Vincent L.} and Stupp, {Samuel I.}",

year = "2011",

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AU - Soukasene, Stephen

AU - Toft, Daniel J.

AU - Moyer, Tyson J.

AU - Lu, Hsuming

AU - Lee, Hyung Kun

AU - Standley, Stephany M.

AU - Cryns, Vincent L.

AU - Stupp, Samuel I.

PY - 2011/11/22

Y1 - 2011/11/22

N2 - Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

AB - Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

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