Apparatus for X-ray diffraction microscopy and tomography of cryo specimens

T. Beetz*, M. R. Howells, C. Jacobsen, C. C. Kao, J. Kirz, E. Lima, T. O. Mentes, H. Miao, C. Sanchez-Hanke, D. Sayre, D. Shapiro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

An apparatus for diffraction microscopy of biological and materials science specimens is described. In this system, a coherent soft X-ray beam is selected with a pinhole, and the illuminated specimen is followed by an adjustable beamstop and CCD camera to record diffraction data from non-crystalline specimens. In addition, a Fresnel zone plate can be inserted to allow for direct imaging. The system makes use of a cryogenic specimen holder with cryotransfer capabilities to allow frozen hydrated specimens to be loaded. The specimen can be tilted over a range of ±80°for three-dimensional imaging; this is done by computer-controlled motors, enabling automated alignment of the specimen through a tilt series. The system is now in use for experiments in soft X-ray diffraction microscopy.

Original languageEnglish (US)
Pages (from-to)459-468
Number of pages10
JournalNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume545
Issue number1-2
DOIs
StatePublished - Jun 11 2005

Keywords

  • Diffraction microscopy
  • Lensless imaging
  • X-ray imaging
  • X-ray microscopy
  • X-ray tomography

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Fingerprint Dive into the research topics of 'Apparatus for X-ray diffraction microscopy and tomography of cryo specimens'. Together they form a unique fingerprint.

Cite this