@article{7e8d243bf64a4b0fae63579a99fc8f16,
title = "Apparent size and morphology of bacterial microcompartments varies with technique",
abstract = "Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies.",
author = "Kennedy, {Nolan W.} and Hershewe, {Jasmine M.} and Nichols, {Taylor M.} and Roth, {Eric W.} and Wilke, {Charlene D.} and Mills, {Carolyn E.} and Jewett, {Michael C.} and Danielle Tullman-Ercek",
note = "Funding Information: This work was supported by the Army Research Office (grants W911NF-16-1-0169 and W911NF-19-1-0298 to DTE). NWK and TMN received support from the National Science Foundation Graduate Research Fellowship Program (grant DGE-1842165). NWK received additional support through the National Institutes of Health Training Grant (T32GM008449) via the Biotechnology Training Program at Northwestern University. This project was supported in part by a fellowship award awarded to JMH through the National Defense Science and Engineering (NDSEG) Fellowship Program, sponsored by the Air Force Research Laboratory, the Office of Naval Research, and the Army Research Office. JMH received support through the Ryan Fellowship awarded by Northwestern University. MCJ and JMH also gratefully acknowledges the Air Force Research Laboratory Center of Excellence Grant FA8650-15-2-5518, the David and Lucile Packard Foundation, and the Camille Dreyfus Teacher-Scholar Program. This work made use of the BioCryo facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); the MRSEC program (NSF DMR-1720139) at the Materials Research Center; the International Institute for Nanotechnology (IIN); and the State of Illinois, through the IIN. It also made use of the CryoCluster equipment, which has received support from the MRI program (NSF DMR-1229693). These funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors would like to thank Robert Colby, Mark Heinnickel, and Giovanni Pilloni for their helpful thoughts on additional methods to include in this work, and members of the Tull-man-Ercek lab, especially Dr. Svetlana Ikonomova, for helpful comments during the preparation of this manuscript. We also thank Dr. Ben Long of the Australian National University for the useful suggestions regarding the basis of our ultra-thin section observations. Publisher Copyright: {\textcopyright} 2020 Kennedy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2020",
doi = "10.1371/journal.pone.0226395",
language = "English (US)",
volume = "15",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",
}