Application of alpha-methylacyl coenzyme A racemase immunohistochemistry in the diagnosis of prostate cancer: A review

Brian P. Adley, Ximing J Yang*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

33 Scopus citations

Abstract

Alpha-methylacyl coenzyme A racemase (AMACR) is a recently discovered enzyme protein that has been shown to be increased at both the mRNA and protein levels in prostatic adenocarcinoma as compared with normal prostatic tissues. Since its discovery, AMACR has gained wide acceptance for use in the diagnosis of prostatic adenocarcinoma in conjunction with morphology and immunohistochemical staining for basal cell markers. Numerous studies have consistently shown high sensitivity and specificity of AMACR for prostate cancer. This review focuses on AMACR expression in prostate cancer and its morphologic variants, high grade prostatic intraepithelial neoplasia, adenosis and benign conditions of the prostate. In addition, we discuss AMACR expression in other tumors. We also focus on the utility and technical aspects of the now-popular "triple stain" immunohistochemical antibody cocktail, consisting of antibodies to high-molecular-weight keratin, p63 and AMACR. Finally, we emphasize diagnostic pitfalls in the application of AMACR to small, atypical foci of glands seen on prostate needle core biopsy and project future diagnostic as well as clinical applications for the protein.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalAnalytical and Quantitative Cytology and Histology
Volume28
Issue number1
StatePublished - Feb 1 2006

Keywords

  • Alpha-methylacyl coenzyme A racemase
  • Immunohistochemistry
  • Prostate cancer

ASJC Scopus subject areas

  • Anatomy
  • Histology

Fingerprint Dive into the research topics of 'Application of alpha-methylacyl coenzyme A racemase immunohistochemistry in the diagnosis of prostate cancer: A review'. Together they form a unique fingerprint.

Cite this