Application of enzyme-linked immunosorbent assay (ELISA) in diagnosis of allergic bronchopulmonary aspergillosis

Paul A. Greenberger*, Roy Patterson

*Corresponding author for this work

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

IgE and IgG antibodies against Af were measured by ELISA in sera from 15 patients with ABPA and compared with antibody levels in six patients with extrinsic asthma and immediate-type skin reactivity to Af (prick skin test 3+ or 4+) but no other evidence for ABPA. The assay used polyvinyl microhemagglutination plates as a solid phase to adsorb antigens of Af and a double-antibody system of class-specific anti-human globulin and alkaline phosphatase-conjugated goat anti-rabbit globulin. IgE-Af and IgG-Af were significantly greater among patients with ABPA than in patients with asthma, suggesting that this assay may be used as an important diagnostic aid. The advantages of ELISA over radioimmunoassay are discussed in regard to early detection of an indolent pulmonary disease.

Original languageEnglish (US)
Pages (from-to)288-293
Number of pages6
JournalThe Journal of laboratory and clinical medicine
Volume99
Issue number2
StatePublished - Feb 1982

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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