Application of immunomagnetic particles to enzyme-linked immunosorbent assay (ELISA) for improvement of detection sensitivity of hcg

Hsiao Ting Kuo, Jay Z. Yeh, Po Hua Wu, Chii Ming Jiang, Ming Chang Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG antibody to superparamagnetic particle (SPIO-anti hCG antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.

Original languageEnglish (US)
Pages (from-to)377-387
Number of pages11
JournalJournal of Immunoassay and Immunochemistry
Volume33
Issue number4
DOIs
StatePublished - Oct 1 2012

Keywords

  • antibody immunomagnetic particle
  • enzyme-linked immunoassay
  • human chorionic gonadotropin
  • magnetic carrier concentration technology
  • pregnancy test
  • superparamagnetic particle

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology

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