@inbook{e23a16deb107495b8adc85b4b45d789b,
title = "Approaches to studying subnuclear organization and gene-nuclear pore interactions",
abstract = "Many genes in budding yeast Saccharomyces cerevisiae associate with the nuclear pore complex (NPC), which impacts their location within the nucleus and their transcriptional regulation. To understand how eukaryotic genomes are spatially organized, we have used multiple approaches for analyzing the localization and transcription of genes. We have used these approaches to study the role of DNA elements in targeting genomic loci to the NPC and how these interactions regulate transcription, chromatin structure and the spatial organization of the yeast genome. These studies combine yeast molecular genetics with live-cell microscopy and biochemistry. Here, we present detailed protocols for these cytological and molecular approaches.",
keywords = "Chromatin, Chromosome, Confocal microscopy, Nuclear architecture, Nuclear pore complex",
author = "Egecioglu, {Defne Emel} and Agustina D'Urso and Brickner, {Donna Garvey} and Light, {William H.} and Brickner, {Jason H.}",
note = "Funding Information: The authors acknowledge support from NIH grant GM 080484 (J. H. B.), a W.M. Keck Young Scholars in Biomedical Research Award (J. H. B.), Cellular and Molecular Basis of Disease Training Program (institutional predoctoral training grant) 2T32GM008061-31 (A. D.), a Rappaport Award for Research Excellence (W. H. L.), and an American Heart Association Postdoctoral Fellowship 13POST14580066 (D. E. E.). The authors also thank members of the Brickner lab for helpful comments on the chapter.",
year = "2014",
doi = "10.1016/B978-0-12-417160-2.00021-7",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc",
pages = "463--485",
booktitle = "Methods in Cell Biology",
}