Aptamer-based viability impedimetric sensor for viruses

Mahmoud Labib; Anna S. Zamay; Darija Muharemagic; Alexey V. Chechik; John C. Bell; Maxim V. Berezovski

doi:10.1021/ac203412m

Aptamer-based viability impedimetric sensor for viruses

Mahmoud Labib, Anna S. Zamay, Darija Muharemagic, Alexey V. Chechik, John C. Bell, Maxim V. Berezovski^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.

Original language	English (US)
Pages (from-to)	1813-1816
Number of pages	4
Journal	Analytical Chemistry
Volume	84
Issue number	4
DOIs	https://doi.org/10.1021/ac203412m
State	Published - Feb 21 2012
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ac203412m

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title = "Aptamer-based viability impedimetric sensor for viruses",

abstract = "The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.",

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AU - Labib, Mahmoud

AU - Zamay, Anna S.

AU - Muharemagic, Darija

AU - Chechik, Alexey V.

AU - Bell, John C.

AU - Berezovski, Maxim V.

PY - 2012/2/21

Y1 - 2012/2/21

N2 - The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.

AB - The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.

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Aptamer-based viability impedimetric sensor for viruses

Abstract

ASJC Scopus subject areas

UN SDGs

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