Aquarius, a novel gene isolated by gene trapping with an RNA-dependent RNA polymerase motif

Mehran Sam, Wolfgang Wurst, Michael Klüppel, Ou Jin, Henry Heng, Alan Bernstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In a retinoic acid (RA) gene-trap screen of mouse embryonic stem (ES) cells, a novel gene, named Aquarius (Aqr), was identified and characterized. The promoterless lacZ marker was used to trap the genomic locus and to determine the expression pattern of the gene. Aqr transcripts are strongly induced in response to RA in vitro. During embryogenesis, Aqr is expressed in mesoderm, in the neural crest and its target tissues, and in neuroepithelium. Expression was first detected at 8.5 days postcoitum, when neural crest cells are visible at the lateral ridges of the neural plate. The gene-trapped Aqr locus was transmitted through the mouse germ line in three genetic backgrounds. In the F2 generation, the expected mendelian ratio of 1:2:1 was observed in all backgrounds, indicating that homozygous mice are viable. Homozygotes are normal in size and weight and breed normally. The gene trap insertion, however, does not seem to generate a null mutation, because Aqr transcripts are still present in the homozygous mutant animals. The Aqr open reading frame has weak homology to RNA-dependent RNA polymerases (RRPs) of the murine hepatitis viruses and contains an RRP motif. Aqr was mapped to mouse chromosome 2 between regions E5 through F2 by using fluorescence in situ hybridization analysis.

Original languageEnglish (US)
Pages (from-to)304-317
Number of pages14
JournalDevelopmental Dynamics
Volume212
Issue number2
DOIs
StatePublished - Jun 1998

Keywords

  • Expressed Sequence Tag database
  • Gene trap
  • Mesoderm
  • Mouse embryonic stem cell
  • Neural crest

ASJC Scopus subject areas

  • Developmental Biology

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