Abstract
The acidic glycosaminoglycan distribution patterns of the aqueous outflow pathway, the iris-ciliary body, and sclera of the New Zealand Red rabbit were identified by analysis of the glycosaminoglycan moieties and by the use of zone electorphoresis. Alcian blue positive bands on cellulose acetate membranes were characterized by a determination of their electrophoretic mobility in two electrolyte systems and by a comparison to the electrophoretic mobility of standard reference glycosaminoglycans. To verify the identity of each band, specific glycosaminoglycan degrading enzymes were used to remove the glycosaminoglycans. Glycosaminoglycan samples equivalent to 5 μg uronic acid were treated with hyaluronate lyase, testicular hyaluronidase, chondroitin AC lyase and chondroitin ABC lyase. The glycosaminoglycans resistant to chondroitin ABC lyase were treated with nitrous acid and tested for solubility in cetyl pyridinium chloride. The scleral distribution pattern was hyaluronic acid, chondroitin sulfate and hybrid dermatan sulfate-chondroitin sulfate. The aqueous outflow pathway and iris-ciliary body distribution patterns were hyaluronic acid, keratan sulfate, heparan sulfate and hybrid dermatan sulfate-chondroitin sulfate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 265-277 |
| Number of pages | 13 |
| Journal | Experimental eye research |
| Volume | 32 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1981 |
Keywords
- aqueous outflow pathway
- chondroitin sulfate
- chondroitinase
- dermatan sulfate
- glycosaminoglycans
- heparan sulfate
- hyaluronic acid
- hyaluronidase
- iris-ciliary body
- keratan sulfate
- proteoglycans
- trabecular meshwork
- zone electrophoresis
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience