Are undifferentiated PC12 rat pheochromocytoma cells electrically excitable?

Damir Janigro*, Gianmaria Maccaferri, Jacopo Meldolesi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Undifferentiated rat pheochromocytoma PC12 cells were current clamped using the whole cell technique. Measurements of cell membrane resting potentials (RMP) gave values in the -30 and -50 mV range. Cell input resistance was between 200 and 400 Mohm. After blockade of K+ currents with intracellular Cs+, cell membrane depolarization showed that PC12 cells are able to generate active responses (i.e., calcium action potentials followed by after-hyperpolarizations partially blocked by tetraethylammonium). Taken together, our results indicate that PC12 cells do not require exposure to nerve growth factor to become electrically excitable.

Original languageEnglish (US)
Pages (from-to)810-814
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume163
Issue number2
DOIs
StatePublished - Sep 15 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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