Undifferentiated rat pheochromocytoma PC12 cells were current clamped using the whole cell technique. Measurements of cell membrane resting potentials (RMP) gave values in the -30 and -50 mV range. Cell input resistance was between 200 and 400 Mohm. After blockade of K+ currents with intracellular Cs+, cell membrane depolarization showed that PC12 cells are able to generate active responses (i.e., calcium action potentials followed by after-hyperpolarizations partially blocked by tetraethylammonium). Taken together, our results indicate that PC12 cells do not require exposure to nerve growth factor to become electrically excitable.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 15 1989|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology