Abstract
Asbestos causes pulmonary fibrosis and various malignancies by mechanisms that remain uncertain. Reactive oxygen species in part cause asbestos toxicity. However, it is not known whether asbestos-induced free radical production causes alveolar epithelial cell (AEC) cytotoxicity by inducing DNA strand breaks (DNA-SB). We tested the hypothesis that asbestos-induced AEC injury in vitro is due to iron-catalyzed free radical generation, which in turn causes DNA-SB. We found that amosite asbestos damages cultured human pulmonary epithelial-like cells (WI-26 cells) as assessed by 51Cr release and that an iron chelator, phytic acid (500 μM), attenuates these effects. A role for iron causing these effects was supported by the observation that ferric chloride-treated phytic acid did not diminish WI-26 cell injury. Production of hydroxyl radical-like species (·OH) was assessed based upon the ·OH-dependent formation of formaldehyde (HCHO) in the presence of dimethyl sulfoxide. A variety of mineral dusts induced significant levels of ·OH formation (nmol HCHO at 30 min: carbonyl iron, 85 ± 21; amosite asbestos, 14 ± 2; chrysotile asbestos, 7 ± 1; titanium dioxide, 2.5 ± 0.5). Phytic acid significantly diminished the asbestos-induced ·OH production. DNA damage to AEC was assessed by the alkaline unwinding, ethidium bromide fluorometric technique. Hydrogen peroxide caused dose- dependent DNA-SB in WI-26 cells after a 30-min exposure period [50% effective dose (ED50): 5 μM] that was similar to other cell lines. Amosite asbestos induced dose-dependent DNA-SB in WI-26, A549, and primary isolated rat alveolar type II cells maintained in culture for 7-10 days (alveolar type I- like). Lower doses of amosite (0.5-5 μg/ml or 0.25-2.5 μg/cm2) caused significant WI-26 cell DNA-SB after prolonged exposure periods (≥ 2 days). Phytic acid ameliorated DNA damage in all three cultured AEC. There was a direct correlation between mineral dust-induced ·OH production at 30 min and DNA-SB in WI-26 cells at 4 h (P < 0.0005). These data suggest that mineral dusts can be directly genotoxic to relevant target cells of asbestos, AEC. Furthermore, these results provide additional support for the premise that iron-catalyzed free radicals mediate asbestos-induced pulmonary toxicity.
Original language | English (US) |
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Pages (from-to) | L471-L480 |
Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
Volume | 268 |
Issue number | 3 12-3 |
DOIs | |
State | Published - 1995 |
Keywords
- cytotoxicity
- deoxyribonucleic acid damage
- reactive oxygen species
ASJC Scopus subject areas
- Physiology
- Pulmonary and Respiratory Medicine
- Physiology (medical)
- Cell Biology