阿司匹林通过ERK信号通路诱导H1299细胞凋亡

Translated title of the contribution: Aspirin Induces Apoptosis in H1299 Cells via ERK Signaling Pathway

Xiang Yu Zhang, Jia Chen, Zi Chun Hua*, Hong Qin Zhuang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the effects of Aspirin on proliferation,migration and apoptosis of H1299 cells and its relevant mechanism,in order to provide the experimental and theoretical basis for clinical prevention and treatment of non-small cell lung cancer(NSCLC).In this study,the effects of Aspirin on the proliferation of H1299 cells were observed by CCK8 assay and 5-ethynyl-2'deoxyuridine (EDU) assay.The effect of Aspirin on apoptosis of H1299 cells was detected by flow cytometry.The cell morphology of H1299 cells after drug treatment was observed under microscope,and the effect of Aspirin on the migration of H1299 cells was detected by Transwell assay.Levels of ERK,p-ERK,Raf and p-Raf protein expression were measured by Western blot.The results of CCK8 experiments showed that Aspirin had a significant inhibitory effect on the growth and proliferation of H1299 cells,and it had a dose-effect relationship.After treatment with 8 mmol/L Aspirin,the proportion of EdU-positive cells decreased significantly (P<0.01),which was consistent with the results of CCK8 experiments.Flow cytometry showed that the proportion of apoptotic cells after Aspirin treatment increased (P<0.01),indicating that Aspirin can induce apoptosis of H1299 cells.The typical morphological changes of apoptosis and migration inhibition were observed under microscope,and it was found that Aspirin reduced the number of H1299 cells passing through the Transwell chamber (P<0.01),indicating that Aspirin can inhibit H1299 cell migration.The authors explored its deeper mechanism and found that the phosphorylation levels of ERK and Raf proteins were down-regulated significantly (P<0.01),but the expression of ERK and Raf protein had no change.In conclusion,Aspirin mainly inhibited proliferation,migration and induced apoptosis of H1299 cells through ERK signaling pathway.Thereby inhibiting the ERK signaling pathway may be one of the prevention and treatment mechanism of non-small cell lung cancer.This experiment provides a theoretical basis for the prevention and treatment of non-small cell lung cancer by Aspirin.

Translated title of the contributionAspirin Induces Apoptosis in H1299 Cells via ERK Signaling Pathway
Original languageChinese (Traditional)
Pages (from-to)189-193
Number of pages5
JournalChinese Journal of Pharmaceutical Biotechnology
Volume26
Issue number3
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

Keywords

  • Apoptosis
  • Aspirin
  • Cell proliferation
  • ERK
  • Migration
  • NSCLC

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

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