Abstract
The assay development for detection and quantitation of fatty acid acyl conjugates of hydrocortisone (HC) is discussed. The gas chromatography-electron capture negative ion mass spectrometry (GC-ECNI-MS) techniques are used for the purpose. Fatty acid acylation of HC would alter the partitioning properties of the glucocorticoid making it more lipid-soluble and more likely to be cell-associated. Deacylation of the fatty acid conjugates of HC would also provide a mechanism through which the biological effect of the endogenous steroid may be prolonged. The sequence of fatty acid acylation and deacylation of HC in vivo may represent a mechanism through which HC modulates inflammation.
Original language | English (US) |
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Title of host publication | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
Pages | 481-482 |
Number of pages | 2 |
State | Published - Dec 1 2002 |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy