Assembly of amorphous clusters under floating monolayers: A comparison of in situ and ex situ techniques

Ahmet Uysal*, Benjamin Stripe, Binhua Lin, Mati Meron, Pulak Dutta

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We report synchrotron X-ray scattering studies of biomimetic crystallization of hydroxyapatite (the primary constituent of bone), using monolayers of fatty acid molecules floating on simulated body fluid (SBF) as well as aqueous solutions of calcium phosphate. A ∼10 Å thick film of amorphous material is observed to form immediately at the molecular monolayer, consistent with the proposed formation of "Posner clusters". This layer becomes denser but not significantly thicker as the subphase concentration and the temperature approach physiological conditions. The amorphous films do not crystallize within 24 h, in contrast to prior reports of more rapid crystallization using electron microscopy on ex situ samples. However, crystallization occurs almost immediately after our films are transferred onto solid substrates. These results illustrate the importance of in situ measurements for model biomineralization experiments.

Original languageEnglish (US)
Pages (from-to)14361-14368
Number of pages8
JournalLangmuir
Volume29
Issue number47
DOIs
StatePublished - Nov 26 2013

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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