Assessment of the stability of an immunoadsorbent for the extracorporeal removal of beta-2-microglobulin from blood

Cynthia M. Daniels, Emily M. Woolverton, Stuart M. Sprague, Guillermo A. Ameer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Dialysis-related amyloidosis (DRA) is a devastating and costly condition that affects patients with end stage kidney disease. A key feature of DRA is the formation of amyloid fibrils, consisting primarily of β2-microglobulin. Except for kidney transplantation, conventional kidney replacement therapies, which are based on nonspecific mechanisms, do not adequately address β2-microglobulin removal. An antihuman β2-microglobulin single-chain variable region antibody fragment (scFv) was developed to confer specificity to β2-microglobulin removal during hemodialysis. Methods: The scFv was immobilized onto agarose and characterized for β2m binding capacity, thermal stability at 37°C, regeneration capacity, storage conditions, and sterility. Results: The β2-microglobulin binding capacity was 1.3 mg/ ml scFv gel. The immunoadsorbent is thermally stable, can be regenerated, stored short-term in 20% ethanol, lyophilized for long-term storage, and withstand process conditions similar to that of a patient's hemodialysis therapy. Conclusions: The results support further investigation of immobilized scFvs as a novel tool to remove β2-microglobulin from blood.

Original languageEnglish (US)
Pages (from-to)287-297
Number of pages11
JournalBlood Purification
Volume23
Issue number4
DOIs
StatePublished - 2005

Keywords

  • Beta-2-microglobulin
  • Dialysis-related amyloidosis
  • End-stage kidney disease

ASJC Scopus subject areas

  • Hematology
  • Nephrology

Fingerprint Dive into the research topics of 'Assessment of the stability of an immunoadsorbent for the extracorporeal removal of beta-2-microglobulin from blood'. Together they form a unique fingerprint.

Cite this