Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA

Gary L. Gallia, Nune Darbinian, Anna Tretiakova, Sameer A. Ansari, Jay Rappaport, John Brady, Margaret J. Wortman, Edward M. Johnson, Kamel Khalili*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The interaction between two regulatory proteins plays a crucial role in the control of several biological events, including gene transcription. In this report, we demonstrate that the interaction between the cellular sequence-specific single-stranded DNA binding protein Purα and the HIV type 1 (HIV-1) Tat protein is mediated by specific ribonucleic acids. The region of Tat that is important for its interaction with Purα includes the region demonstrated to bind Tat's viral RNA target, TAR. A 10-nucleotide GC-rich consensus sequence identified in RNAs associated with Purα derived from human U-87MG cells plays an important role in the Purα:Tat interaction as examined by an in vitro reconstitution assay. Furthermore; expression of the Purα-associated RNA in these cells enhances transcriptional activation of the HIV-1 promoter by Tat and Purα.

Original languageEnglish (US)
Pages (from-to)11572-11577
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number20
DOIs
StatePublished - Sep 28 1999

ASJC Scopus subject areas

  • General

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