Association of MBP peptides with Hsp70 in normal appearing human white matter

Brett T. Lund*, Yervand Chakryan, Nazely Ashikian, Lilit Mnatsakanyan, Carolyn J. Bevan, Rodrigo Aguilera, Tim Gallaher, Michael W. Jakowec

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Multiple Sclerosis is an autoimmune disease directed against myelin proteins. The etiology of MS is poorly defined though, with no definitive causative agent yet identified. It has been hypothesized that MS may be a multifactorial disease resulting in the same end product: the destruction of myelin by the immune system. In this report we describe a potential role for heat shock proteins in the pathogenesis of MS. We isolated Hsp70 from the normal appearing white matter of both MS and normal human brain and found this was actively associated with, among other things, immunodominant MBP peptides. Hsp70-MBP peptide complexes prepared in vitro were shown to be highly immunogenic, with adjuvant-like effects stimulating MBP peptide-specific T cell lines to respond to normally sub-optimal concentrations of peptide. This demonstration of a specific interaction between Hsp70 and different MBP peptides, coupled with the adjuvanticity of this association is suggestive of a possible role for Hsp70 in the immunopathology associated with MS.

Original languageEnglish (US)
Pages (from-to)122-134
Number of pages13
JournalJournal of the Neurological Sciences
Volume249
Issue number2
DOIs
StatePublished - Nov 15 2006

Keywords

  • Adjuvant
  • Autoimmune
  • Hsp70
  • Immune response
  • MBP
  • Multiple Sclerosis

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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