Associations between functional polymorphisms in antioxidant defense genes and urinary oxidative stress biomarkers in healthy, premenopausal women

Umaima Al-Alem, Peter H. Gann, Jeffrey Dahl, Richard B. Van Breemen, Vilas Mistry, Patricia M.W. Lam, Mark D. Evans, Linda Van Horn, Margaret E. Wright*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Functional polymorphisms in endogenous antioxidant defense genes including manganese superoxide dismutase (MnSOD), catalase (CAT), and glutathione peroxidase (GPX-1) have been linked with risk of cancer at multiple sites. Although it is presumed that these germline variants impact disease risk by altering the host's ability to detoxify mutagenic reactive oxygen species, very few studies have directly examined this hypothesis. Concentrations of 8-isoprostane F2a (8-iso-PGF2α) and 8-oxo-7, 8-dihydro-2″- deoxyguanosine (8-oxoxdG)-sensitive indicators of lipid peroxidation and DNA oxidation, respectively- were measured in 24-h urine samples obtained from 93 healthy, premenopausal women participating in a dietary intervention trial. In addition, DNA was extracted from blood for genotyping of MnSOD Val16Ala, CAT-262 C>T, and GPX1 Pro198Leu genotypes by Taqman assay. Although geometric mean concentrations of 8-iso-PGF2a and 8-oxoxdG varied across several study characteristics including race, education level, body mass index, and serum antioxidant levels, there was little evidence that these biomarkers differed across any of the examined genotypes. In summary, functional polymorphisms in endogenous antioxidant defense genes do not appear to be strongly associated with systemic oxidative stress levels in young, healthy women.

Original languageEnglish (US)
Pages (from-to)191-195
Number of pages5
JournalGenes and Nutrition
Volume7
Issue number2
DOIs
StatePublished - Apr 2012

Keywords

  • Antioxidant
  • Biomarker
  • Oxidative stress
  • Polymorphism
  • Women

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Genetics

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