Associations of sex, age and adiposity in endothelium-independent dilation in children

Michelle M. Harbin; Hanan Zavala; Justin R. Ryder; Julia Steinberger; Alan R. Sinaiko; David R. Jacobs; Donald R. Dengel

doi:10.1088/1361-6579/aab4cf

Associations of sex, age and adiposity in endothelium-independent dilation in children

Michelle M. Harbin, Hanan Zavala, Justin R. Ryder, Julia Steinberger, Alan R. Sinaiko, David R. Jacobs, Donald R. Dengel

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Abstract

Objective: To examine the association of age, sex, and obesity status on endothelial-independent dilation (EID) among children and adolescents. Study design: This study examined 264 children (143 males) between 8 to 18 years old (mean ± SD: Age = 14.3 ± 2.7 years). Endothelial-independent dilation was assessed via ultrasound imaging of the brachial artery following administration of 0.3 milligrams of sublingual nitroglycerin. A one-way analysis of variance with Bonferroni post hoc comparisons assessed sex-differences in percent peak EID dilation (EID%-peak) and EID area under the curve (EID%-AUC), while analysis of covariance (ANCOVA) adjusted for race, age, body mass index percentile (BMI-percentile), and brachial artery diameter. Multiple linear regression evaluated the association of sex, age, BMI-percentile, percent body fat (%BF), and brachial artery diameter on EID. Results: Prior to adjustment, EID%-peak was significantly higher among females than males (mean ± SE: 26.9 ± 0.5% versus 22.9 ± 0.6%, p < 0.001, respectively); similar findings for EID%-AUC (4214% ± 105%•s versus 3398% ± 97%•s, p < 0.001) were observed. After adjusting for covariates, EID%-AUC remained consistent (p = 0.03) while EID%-peak was not significantly different between sexes (p = 0.21). EID%-peak was significantly higher among normal weight compared to obese participants (p = 0.04), while no differences were observed between obesity status after adjustment for brachial artery diameter (p = 0.64). Both unadjusted (p = 0.16) and adjusted EID%-AUC (p = 0.24) was not significantly different between obesity status. BMI-percentile was not associated with EID%-peak (p = 0.76) or EID%-AUC (p = 0.30). Additionally, %BF was not associated with EID%-peak (p = 0.56) or EID%-AUC (p = 0.15). After adjusting for brachial artery diameter, BMI-percentile, and age, males had lower EID%-AUC (p = 0.03) and lower but not significant EID%-peak (p = 0.21). Significance: Vascular smooth muscle function was significantly lower among male children and adolescents, which is suggestive that impaired EID and increased cardiovascular disease risk among males may begin in childhood. Interestingly, obesity status and BMI-percentile was not associated with EID in children and adolescents after adjusting for brachial artery diameter.

Original language	English (US)
Article number	045002
Journal	Physiological Measurement
Volume	39
Issue number	4
DOIs	https://doi.org/10.1088/1361-6579/aab4cf
State	Published - Apr 20 2018

Funding

This project is supported by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS), NCI/NIDDK (R01CA113930-01A1 to JS), General Clinical Research Center Program (M01-RR00400), National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114),and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR). The authors declare no conflicts of interest. HZ wrote the first draft of the manuscript. No payment was received to produce this manuscript.All authors have seen,approved,and take full responsibility for the manuscript.This project is support by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS),NCI/NIDDK (R01CA113930-01A1 to JS),General Clinical Research Center Program (M01-RR00400),National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114), and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR).

Keywords

body mass index
cardiovascular disease risk
pediatric
Vascular smooth muscle function

ASJC Scopus subject areas

Biophysics
Physiology
Biomedical Engineering
Physiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1088/1361-6579/aab4cf

Cite this

@article{43e85484d249402c853d0b7307082909,

title = "Associations of sex, age and adiposity in endothelium-independent dilation in children",

abstract = "Objective: To examine the association of age, sex, and obesity status on endothelial-independent dilation (EID) among children and adolescents. Study design: This study examined 264 children (143 males) between 8 to 18 years old (mean ± SD: Age = 14.3 ± 2.7 years). Endothelial-independent dilation was assessed via ultrasound imaging of the brachial artery following administration of 0.3 milligrams of sublingual nitroglycerin. A one-way analysis of variance with Bonferroni post hoc comparisons assessed sex-differences in percent peak EID dilation (EID%-peak) and EID area under the curve (EID%-AUC), while analysis of covariance (ANCOVA) adjusted for race, age, body mass index percentile (BMI-percentile), and brachial artery diameter. Multiple linear regression evaluated the association of sex, age, BMI-percentile, percent body fat (%BF), and brachial artery diameter on EID. Results: Prior to adjustment, EID%-peak was significantly higher among females than males (mean ± SE: 26.9 ± 0.5% versus 22.9 ± 0.6%, p < 0.001, respectively); similar findings for EID%-AUC (4214% ± 105%•s versus 3398% ± 97%•s, p < 0.001) were observed. After adjusting for covariates, EID%-AUC remained consistent (p = 0.03) while EID%-peak was not significantly different between sexes (p = 0.21). EID%-peak was significantly higher among normal weight compared to obese participants (p = 0.04), while no differences were observed between obesity status after adjustment for brachial artery diameter (p = 0.64). Both unadjusted (p = 0.16) and adjusted EID%-AUC (p = 0.24) was not significantly different between obesity status. BMI-percentile was not associated with EID%-peak (p = 0.76) or EID%-AUC (p = 0.30). Additionally, %BF was not associated with EID%-peak (p = 0.56) or EID%-AUC (p = 0.15). After adjusting for brachial artery diameter, BMI-percentile, and age, males had lower EID%-AUC (p = 0.03) and lower but not significant EID%-peak (p = 0.21). Significance: Vascular smooth muscle function was significantly lower among male children and adolescents, which is suggestive that impaired EID and increased cardiovascular disease risk among males may begin in childhood. Interestingly, obesity status and BMI-percentile was not associated with EID in children and adolescents after adjusting for brachial artery diameter.",

keywords = "body mass index, cardiovascular disease risk, pediatric, Vascular smooth muscle function",

author = "Harbin, {Michelle M.} and Hanan Zavala and Ryder, {Justin R.} and Julia Steinberger and Sinaiko, {Alan R.} and Jacobs, {David R.} and Dengel, {Donald R.}",

note = "Funding Information: This project is supported by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS), NCI/NIDDK (R01CA113930-01A1 to JS), General Clinical Research Center Program (M01-RR00400), National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114),and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR). Funding Information: The authors declare no conflicts of interest. HZ wrote the first draft of the manuscript. No payment was received to produce this manuscript.All authors have seen,approved,and take full responsibility for the manuscript.This project is support by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS),NCI/NIDDK (R01CA113930-01A1 to JS),General Clinical Research Center Program (M01-RR00400),National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114), and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR). Publisher Copyright: {\textcopyright} 2018 Institute of Physics and Engineering in Medicine.",

year = "2018",

month = apr,

day = "20",

doi = "10.1088/1361-6579/aab4cf",

language = "English (US)",

volume = "39",

journal = "Physiological Measurement",

issn = "0967-3334",

publisher = "IOP Publishing Ltd.",

number = "4",

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TY - JOUR

T1 - Associations of sex, age and adiposity in endothelium-independent dilation in children

AU - Harbin, Michelle M.

AU - Zavala, Hanan

AU - Ryder, Justin R.

AU - Steinberger, Julia

AU - Sinaiko, Alan R.

AU - Jacobs, David R.

AU - Dengel, Donald R.

N1 - Funding Information: This project is supported by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS), NCI/NIDDK (R01CA113930-01A1 to JS), General Clinical Research Center Program (M01-RR00400), National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114),and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR). Funding Information: The authors declare no conflicts of interest. HZ wrote the first draft of the manuscript. No payment was received to produce this manuscript.All authors have seen,approved,and take full responsibility for the manuscript.This project is support by funding from the NIH/NIDDK (R01-DK072124-01A3 to JS),NCI/NIDDK (R01CA113930-01A1 to JS),General Clinical Research Center Program (M01-RR00400),National Center for Research Resources (1UL1-RR033183), the Clinical and Translational Science Institute at the University of Minnesota-Twin Cities (UL1TR000114), and an individual training grant from NIH/NHLBI (F32-HL127851-01 to JRR). Publisher Copyright: © 2018 Institute of Physics and Engineering in Medicine.

PY - 2018/4/20

Y1 - 2018/4/20

N2 - Objective: To examine the association of age, sex, and obesity status on endothelial-independent dilation (EID) among children and adolescents. Study design: This study examined 264 children (143 males) between 8 to 18 years old (mean ± SD: Age = 14.3 ± 2.7 years). Endothelial-independent dilation was assessed via ultrasound imaging of the brachial artery following administration of 0.3 milligrams of sublingual nitroglycerin. A one-way analysis of variance with Bonferroni post hoc comparisons assessed sex-differences in percent peak EID dilation (EID%-peak) and EID area under the curve (EID%-AUC), while analysis of covariance (ANCOVA) adjusted for race, age, body mass index percentile (BMI-percentile), and brachial artery diameter. Multiple linear regression evaluated the association of sex, age, BMI-percentile, percent body fat (%BF), and brachial artery diameter on EID. Results: Prior to adjustment, EID%-peak was significantly higher among females than males (mean ± SE: 26.9 ± 0.5% versus 22.9 ± 0.6%, p < 0.001, respectively); similar findings for EID%-AUC (4214% ± 105%•s versus 3398% ± 97%•s, p < 0.001) were observed. After adjusting for covariates, EID%-AUC remained consistent (p = 0.03) while EID%-peak was not significantly different between sexes (p = 0.21). EID%-peak was significantly higher among normal weight compared to obese participants (p = 0.04), while no differences were observed between obesity status after adjustment for brachial artery diameter (p = 0.64). Both unadjusted (p = 0.16) and adjusted EID%-AUC (p = 0.24) was not significantly different between obesity status. BMI-percentile was not associated with EID%-peak (p = 0.76) or EID%-AUC (p = 0.30). Additionally, %BF was not associated with EID%-peak (p = 0.56) or EID%-AUC (p = 0.15). After adjusting for brachial artery diameter, BMI-percentile, and age, males had lower EID%-AUC (p = 0.03) and lower but not significant EID%-peak (p = 0.21). Significance: Vascular smooth muscle function was significantly lower among male children and adolescents, which is suggestive that impaired EID and increased cardiovascular disease risk among males may begin in childhood. Interestingly, obesity status and BMI-percentile was not associated with EID in children and adolescents after adjusting for brachial artery diameter.

AB - Objective: To examine the association of age, sex, and obesity status on endothelial-independent dilation (EID) among children and adolescents. Study design: This study examined 264 children (143 males) between 8 to 18 years old (mean ± SD: Age = 14.3 ± 2.7 years). Endothelial-independent dilation was assessed via ultrasound imaging of the brachial artery following administration of 0.3 milligrams of sublingual nitroglycerin. A one-way analysis of variance with Bonferroni post hoc comparisons assessed sex-differences in percent peak EID dilation (EID%-peak) and EID area under the curve (EID%-AUC), while analysis of covariance (ANCOVA) adjusted for race, age, body mass index percentile (BMI-percentile), and brachial artery diameter. Multiple linear regression evaluated the association of sex, age, BMI-percentile, percent body fat (%BF), and brachial artery diameter on EID. Results: Prior to adjustment, EID%-peak was significantly higher among females than males (mean ± SE: 26.9 ± 0.5% versus 22.9 ± 0.6%, p < 0.001, respectively); similar findings for EID%-AUC (4214% ± 105%•s versus 3398% ± 97%•s, p < 0.001) were observed. After adjusting for covariates, EID%-AUC remained consistent (p = 0.03) while EID%-peak was not significantly different between sexes (p = 0.21). EID%-peak was significantly higher among normal weight compared to obese participants (p = 0.04), while no differences were observed between obesity status after adjustment for brachial artery diameter (p = 0.64). Both unadjusted (p = 0.16) and adjusted EID%-AUC (p = 0.24) was not significantly different between obesity status. BMI-percentile was not associated with EID%-peak (p = 0.76) or EID%-AUC (p = 0.30). Additionally, %BF was not associated with EID%-peak (p = 0.56) or EID%-AUC (p = 0.15). After adjusting for brachial artery diameter, BMI-percentile, and age, males had lower EID%-AUC (p = 0.03) and lower but not significant EID%-peak (p = 0.21). Significance: Vascular smooth muscle function was significantly lower among male children and adolescents, which is suggestive that impaired EID and increased cardiovascular disease risk among males may begin in childhood. Interestingly, obesity status and BMI-percentile was not associated with EID in children and adolescents after adjusting for brachial artery diameter.

KW - body mass index

KW - cardiovascular disease risk

KW - pediatric

KW - Vascular smooth muscle function

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Associations of sex, age and adiposity in endothelium-independent dilation in children

Abstract

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Keywords

ASJC Scopus subject areas

UN SDGs

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