TY - JOUR
T1 - Astroglial and cognitive effects of chronic cerebral hypoperfusion in the rat
AU - Vicente, Évelin
AU - Degerone, Daniel
AU - Bohn, Liana
AU - Scornavaca, Francisco
AU - Pimentel, Alexandre
AU - Leite, Marina C.
AU - Swarowsky, Alessandra
AU - Rodrigues, Letícia
AU - Nardin, Patrícia
AU - Vieira de Almeida, Lucia Maria
AU - Gottfried, Carmem
AU - Souza, Diogo Onofre
AU - Netto, Carlos Alexandre
AU - Gonçalves, Carlos Alberto
N1 - Funding Information:
Brazilian funds from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES), FINEP/Rede IBN 01.06.0842-00.
PY - 2009/1/28
Y1 - 2009/1/28
N2 - The permanent occlusion of common carotid arteries (2VO) causes a significant reduction of cerebral blood flow (hypoperfusion) in rats and constitutes a well established experimental model to investigate neuronal damage and cognitive impairment that occurs in human ageing and Alzheimer's disease. In the present study, we evaluated two astroglial proteins - S100B and glial fibrillary acidic protein (GFAP) - in cerebral cortex and hippocampus tissue, glutamate uptake and glutamine synthetase activity in hippocampus tissue, as well as S100B in cerebrospinal fluid. Cognition, as assessed by reference and working spatial memory protocols, was also investigated. Adult male Wistar rats were submitted to 10 weeks of chronic cerebral hypoperfusion by the 2VO method. A significant increase of S100B and GFAP in hippocampus tissue was observed, as well a significant decrease in glutamate uptake. Interestingly, we observed a decrease in S100B in cerebrospinal fluid. As for the cognitive outcome, there was an impairment of both reference and working spatial memory in the water maze; positive correlation between cognitive impairment and glutamate uptake decrease was evidenced in hypoperfused rats. These data support the hypothesis that astrocytes play a crucial role in the mechanisms of experimental neurodegeneration and that hippocampal pathology arising after chronic hypoperfusion gives rise to memory deficits.
AB - The permanent occlusion of common carotid arteries (2VO) causes a significant reduction of cerebral blood flow (hypoperfusion) in rats and constitutes a well established experimental model to investigate neuronal damage and cognitive impairment that occurs in human ageing and Alzheimer's disease. In the present study, we evaluated two astroglial proteins - S100B and glial fibrillary acidic protein (GFAP) - in cerebral cortex and hippocampus tissue, glutamate uptake and glutamine synthetase activity in hippocampus tissue, as well as S100B in cerebrospinal fluid. Cognition, as assessed by reference and working spatial memory protocols, was also investigated. Adult male Wistar rats were submitted to 10 weeks of chronic cerebral hypoperfusion by the 2VO method. A significant increase of S100B and GFAP in hippocampus tissue was observed, as well a significant decrease in glutamate uptake. Interestingly, we observed a decrease in S100B in cerebrospinal fluid. As for the cognitive outcome, there was an impairment of both reference and working spatial memory in the water maze; positive correlation between cognitive impairment and glutamate uptake decrease was evidenced in hypoperfused rats. These data support the hypothesis that astrocytes play a crucial role in the mechanisms of experimental neurodegeneration and that hippocampal pathology arising after chronic hypoperfusion gives rise to memory deficits.
KW - 2-VO method
KW - Astrocyte
KW - GFAP
KW - Hippocampus
KW - Hypoperfusion
KW - S100B
UR - http://www.scopus.com/inward/record.url?scp=58149389909&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149389909&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2008.11.032
DO - 10.1016/j.brainres.2008.11.032
M3 - Article
C2 - 19056357
AN - SCOPUS:58149389909
SN - 0006-8993
VL - 1251
SP - 204
EP - 212
JO - Brain research
JF - Brain research
ER -