Asynchronous expression of glucose-6-phosphate dehydrogenase in splake trout embryos

Toshio Yamauchi*, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Electrophoretic analysis of the developmental progression of isozymes provides a sensitive method for observing the expression of genes. Glucose-6-phosphate dehydrogenase (G-6-PD) activity profiles and isozyme patterns were examined in developing brook trout (Salvelinus fontinalis), lake trout (Salvelinus namaycush), and their viable hybrid, splake trout. In all crosses examined, the G-6-PD level of trout embryos dropped immediately upon fertilization and then steadily increased after completion of gastrulation. In the F1 cross, brook ♀×lake ♂, the maternal isozyme pattern persisted until 1 week after completion of yolk resorption. In F2 crosses, F1 splake ♀×F1 splake ♂, at hatching all embryos revealed either a brook or lake G-6-PD pattern. Thus, stable products from the oocyte (splake pattern) were no longer present, and the preferential expression of one parental gene (probably maternal) was demonstrated. In addition, the hybrid pattern was first detected 2 weeks before yolk resorption. This asynchrony in gene expression reflects incompatability between the maternal protein synthesizing machinery and the paternal genome. Perhaps it involves the recognition of more of the paternal genome than just the structural gene loci. Since the F1 hybrid pattern was further delayed until after yolk resorption, suppression by a component in the yolk may also be involved.

Original languageEnglish (US)
Pages (from-to)63-68
Number of pages6
JournalDevelopmental Biology
Volume39
Issue number1
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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