AtJ6, a unique J-domain protein from Arabidopsis thaliana

Barbara Kroczyńska, Nichole E. Coop, Jan A. Miernyk

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An Arabidopsis thaliana cDNA encoding a protein, AtJ6, related to Escherichia coli DnaJ was sequenced. Translation of the At J6 nucleotide sequence yields a protein with an N-terminal J-domain, but which lacks the G/F, and C-rich domains characteristic of DnaJ. In addition to the J-domain, one region of the sequence has homology with the prokaryotic FtsA protein. The remainder of the At J6 sequence is not notably related to anything in the sequence databases. The At J6 sequence represents a unique member of the A. thaliana J-domain protein family. The results of Northern analysis revealed that the At J6 mRNA is expressed in all organs of A. thaliana; expression was high in leaves, flowers, and siliques, and low in roots. The results of Southern analysis are consistent with a single unique gene.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalPlant Science
Volume151
Issue number1
DOIs
StatePublished - Feb 7 2000

Keywords

  • Chaperone
  • Exon
  • Expression
  • Gene
  • Intron
  • Nucleotide sequence
  • cDNA

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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