Adenosine 5'-triphosphate (ATP, 0.1-100 μM), produced inward currents in patch-clamped coeliac neurones from guinea-pig when studied in either the whole cell configuration or in excised (outside-out) patches. The P2-purinoceptor antagonists suramin (80-230 μM) or reactive blue 2 (2-20 μM) depressed the ATP-induced currents but not those produced by acetylcholine. Excitatory post-synaptic currents (e.p.s.cs) were observed in cultured neurones. E.p.s.cs had similar current-voltage relationships to currents evoked by ATP in excised patches and were reduced by suramin or reactive blue 2 to a similar extent as ATP currents. The results suggest that ATP is the excitatory neurotransmitter in cultures of these neurones.
|Original language||English (US)|
|Number of pages||2|
|Journal||British journal of pharmacology|
|State||Published - Aug 1992|
- Reactive blue 2
- Synaptic currents
ASJC Scopus subject areas