ATP mediates excitatory synaptic transmission in mammalian neurones

E. M. Silinsky*, V. Gerzanich, S. M. Vanner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Adenosine 5'-triphosphate (ATP, 0.1-100 μM), produced inward currents in patch-clamped coeliac neurones from guinea-pig when studied in either the whole cell configuration or in excised (outside-out) patches. The P2-purinoceptor antagonists suramin (80-230 μM) or reactive blue 2 (2-20 μM) depressed the ATP-induced currents but not those produced by acetylcholine. Excitatory post-synaptic currents (e.p.s.cs) were observed in cultured neurones. E.p.s.cs had similar current-voltage relationships to currents evoked by ATP in excised patches and were reduced by suramin or reactive blue 2 to a similar extent as ATP currents. The results suggest that ATP is the excitatory neurotransmitter in cultures of these neurones.

Original languageEnglish (US)
Pages (from-to)762-763
Number of pages2
JournalBritish journal of pharmacology
Volume106
Issue number4
DOIs
StatePublished - Aug 1992

Keywords

  • Acetylcholine
  • ATP
  • Purinergic
  • Reactive blue 2
  • Suramin
  • Synaptic currents

ASJC Scopus subject areas

  • Pharmacology

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