Attachment of anti-GFP antibodies to microspheres for optical trapping experiments

James A. Spudich, Sarah E. Rice, Ronald S. Rock, Thomas J. Purcell, Hans M. Warrick

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes a method for attaching anti-GFP (green fluorescent protein) antibodies to microspheres. GFP-motor fusion proteins can then be adsorbed to the microspheres for use in single-molecule motility studies and optical trapping experiments.

Original languageEnglish (US)
Pages (from-to)1370-1371
Number of pages2
JournalCold Spring Harbor Protocols
Volume6
Issue number11
DOIs
StatePublished - Nov 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Spudich, J. A., Rice, S. E., Rock, R. S., Purcell, T. J., & Warrick, H. M. (2011). Attachment of anti-GFP antibodies to microspheres for optical trapping experiments. Cold Spring Harbor Protocols, 6(11), 1370-1371. https://doi.org/10.1101/pdb.prot066670