Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry

Gary A. Valaskovic; Neil L. Kelleher; Daniel P. Little; David J. Aaserud; Fred W. McLafferty

doi:10.1021/ac00116a030

Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry

Gary A. Valaskovic, Neil L. Kelleher, Daniel P. Little, David J. Aaserud, Fred W. McLafferty^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

211 Scopus citations

Abstract

Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 μm i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 10⁵ for full mass spectra, while the total amount of material consumed is, ~150 and 10 amol, respectively.

Original language	English (US)
Pages (from-to)	3802-3805
Number of pages	4
Journal	Analytical Chemistry
Volume	67
Issue number	20
DOIs	https://doi.org/10.1021/ac00116a030
State	Published - 1995

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac00116a030

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title = "Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry",

abstract = "Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 μm i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 105 for full mass spectra, while the total amount of material consumed is, ~150 and 10 amol, respectively.",

author = "Valaskovic, {Gary A.} and Kelleher, {Neil L.} and Little, {Daniel P.} and Aaserud, {David J.} and McLafferty, {Fred W.}",

year = "1995",

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language = "English (US)",

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pages = "3802--3805",

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T1 - Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry

AU - Valaskovic, Gary A.

AU - Kelleher, Neil L.

AU - Little, Daniel P.

AU - Aaserud, David J.

AU - McLafferty, Fred W.

PY - 1995

Y1 - 1995

N2 - Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 μm i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 105 for full mass spectra, while the total amount of material consumed is, ~150 and 10 amol, respectively.

AB - Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 μm i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 105 for full mass spectra, while the total amount of material consumed is, ~150 and 10 amol, respectively.

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Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry

Abstract

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