Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry

Gary A. Valaskovic, Neil L. Kelleher, Daniel P. Little, David J. Aaserud, Fred W. McLafferty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

210 Scopus citations


Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 μm i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 105 for full mass spectra, while the total amount of material consumed is, ~150 and 10 amol, respectively.

Original languageEnglish (US)
Pages (from-to)3802-3805
Number of pages4
JournalAnalytical Chemistry
Issue number20
StatePublished - 1995

ASJC Scopus subject areas

  • Analytical Chemistry


Dive into the research topics of 'Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry'. Together they form a unique fingerprint.

Cite this