Autoantibodies to DEK oncoprotein in human inflammatory disease

Xingwen Dong, Jingsong Wang, Fathima N. Kabir, Melody Shaw, Ann M. Reed, Leonard Stein, Luis E C Andrade, Virginia F M Trevisani, Michael L. Miller, Takao Fujii, Masashi Akizuki, Lauren M. Pachman, Minoru Satoh, Westley H. Reeves*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA). Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation studies using baculovirus-expressed human DEK. Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21). Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. In general, levels of anti- DEK autoantibodies were higher in SLE than in other patient subsets, including JRA. Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-γ production rather than a particular disease subset.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalArthritis and rheumatism
Volume43
Issue number1
DOIs
StatePublished - Jan 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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