Autofluorescence of live purple bacteria in the near infrared

Guenter Albrecht-Buehler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We have developed a novel microscope with which to study the fluorescence of cells in the near-infrared region (k = 750-2500 nm). For one of its first applications we report on the autofluorescence of live purple bacteria, Rhodospirillum rubrum, and suggest that the autofluorescent component is bacteriochlorophyll. The rapid fading of the autofluorescence of fixed bacteria and of purified bacteriochlorophyll suggests that the live bacteria are able to regenerate their pigment with a time constant of approximately 20 s.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalExperimental Cell Research
Volume236
Issue number1
DOIs
StatePublished - Oct 10 1997

ASJC Scopus subject areas

  • Cell Biology

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