TY - JOUR
T1 - Autofluorescence of live purple bacteria in the near infrared
AU - Albrecht-Buehler, Guenter
N1 - Funding Information:
I am very grateful to Dr. Douglas B. Kell (Institute of Biological Sciences, University of Wales, Aberystwyth). for his idea to study the auto¯uorescence of cells in the infrared. I also thank Ms. Yifei Jiang for her excellent technical assistance. This work was supported by grants from the Air Force Of®ce of Scienti®c Research (Grant F49620-96-1-0051) and the U.S. Army Research Of®ce (Grant DAAH04-95-1-0180).
PY - 1997/10/10
Y1 - 1997/10/10
N2 - We have developed a novel microscope with which to study the fluorescence of cells in the near-infrared region (k = 750-2500 nm). For one of its first applications we report on the autofluorescence of live purple bacteria, Rhodospirillum rubrum, and suggest that the autofluorescent component is bacteriochlorophyll. The rapid fading of the autofluorescence of fixed bacteria and of purified bacteriochlorophyll suggests that the live bacteria are able to regenerate their pigment with a time constant of approximately 20 s.
AB - We have developed a novel microscope with which to study the fluorescence of cells in the near-infrared region (k = 750-2500 nm). For one of its first applications we report on the autofluorescence of live purple bacteria, Rhodospirillum rubrum, and suggest that the autofluorescent component is bacteriochlorophyll. The rapid fading of the autofluorescence of fixed bacteria and of purified bacteriochlorophyll suggests that the live bacteria are able to regenerate their pigment with a time constant of approximately 20 s.
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U2 - 10.1006/excr.1996.3688
DO - 10.1006/excr.1996.3688
M3 - Article
C2 - 9344583
AN - SCOPUS:0031563763
SN - 0014-4827
VL - 236
SP - 43
EP - 50
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -