TY - JOUR
T1 - Automated electrorotation to reveal dielectric variations related to HER-2/neu overexpression in MCF-7 sublines
AU - Cristofanilli, Massimo
AU - De Gasperis, Giovanni
AU - Zhang, Lisha
AU - Hung, Mien Chie
AU - Gascoyne, Peter R C
AU - Hortobagyi, Gabriel N.
PY - 2002
Y1 - 2002
N2 - Purpose: Electrorotation (ROT) is a technique that allows for determination of the dielectric properties of living cells when exposed to a rotating electric field. We evaluated the ROT behavior of MCF/neo and p185neu transfectancts MCF/HER2-11 and MCF/HER2-18 to investigate whether differences in HER-2/neu expression were associated with differences in dielectric properties in these cells. Experimental Design: P185neu was measured by Western blotting in MCF/neo cells and HER-2/neu transfectants MCF/HER2-11 and MCF/HER2-18. ROT spectra and cell membrane-specific capacitance were obtained for each cell line. Results: The mean cell membrane-specific capacitance values for MCF/neo, MCF/HER2-11, and MCF/HER2-18 were 2.09, 1.70, and 2.56 μF/cm2, respectively. The mean specific capacitance for MCF/neo was significantly different from that for MCF/HER2-11 (P = 0.006) and that for MCF/HER2-18 (P = 0.007). Conclusions: ROT is sufficiently sensitive to detect variations in dielectric properties in breast cancer cell lines overexpressing p185neu. These differences may be related to the morphological alterations determined by HER-2/neu overexpression.
AB - Purpose: Electrorotation (ROT) is a technique that allows for determination of the dielectric properties of living cells when exposed to a rotating electric field. We evaluated the ROT behavior of MCF/neo and p185neu transfectancts MCF/HER2-11 and MCF/HER2-18 to investigate whether differences in HER-2/neu expression were associated with differences in dielectric properties in these cells. Experimental Design: P185neu was measured by Western blotting in MCF/neo cells and HER-2/neu transfectants MCF/HER2-11 and MCF/HER2-18. ROT spectra and cell membrane-specific capacitance were obtained for each cell line. Results: The mean cell membrane-specific capacitance values for MCF/neo, MCF/HER2-11, and MCF/HER2-18 were 2.09, 1.70, and 2.56 μF/cm2, respectively. The mean specific capacitance for MCF/neo was significantly different from that for MCF/HER2-11 (P = 0.006) and that for MCF/HER2-18 (P = 0.007). Conclusions: ROT is sufficiently sensitive to detect variations in dielectric properties in breast cancer cell lines overexpressing p185neu. These differences may be related to the morphological alterations determined by HER-2/neu overexpression.
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M3 - Article
C2 - 11839684
AN - SCOPUS:0036184414
SN - 1078-0432
VL - 8
SP - 615
EP - 619
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 2
ER -
