Autoradiography of dendritic acetylcholine receptors: A method for study of isolated neurons of the adult central nervous system in the turtle

William L. James; William L. Klein

doi:10.1016/0304-3940(82)90220-8

Autoradiography of dendritic acetylcholine receptors: A method for study of isolated neurons of the adult central nervous system in the turtle

William L. James, William L. Klein^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Adult neurons were isolated from turtle retina in order to study the subcellular distribution of muscarinic acetylcholine receptors. Retinas were perfused in vitro with [³H]quinuclidinylbenzilate [³H]QNB) to label muscarinic receptors, and individual neurons with extensive dendritic arborization were obtained for autoradiography by use of papain and trituration. Papain caused no change in the number of muscarinic receptor sites nor in their affinity for [³H]QNB. Receptors, indicated by the positions of silver grains, were localized to dendritic processes.

Original language	English (US)
Pages (from-to)	5-10
Number of pages	6
Journal	Neuroscience Letters
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(82)90220-8
State	Published - Sep 20 1982

Keywords

autoradiography
cholinergic
dendrites
muscarinic
receptor localization
retina

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(82)90220-8

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title = "Autoradiography of dendritic acetylcholine receptors: A method for study of isolated neurons of the adult central nervous system in the turtle",

abstract = "Adult neurons were isolated from turtle retina in order to study the subcellular distribution of muscarinic acetylcholine receptors. Retinas were perfused in vitro with [3H]quinuclidinylbenzilate [3H]QNB) to label muscarinic receptors, and individual neurons with extensive dendritic arborization were obtained for autoradiography by use of papain and trituration. Papain caused no change in the number of muscarinic receptor sites nor in their affinity for [3H]QNB. Receptors, indicated by the positions of silver grains, were localized to dendritic processes.",

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T1 - Autoradiography of dendritic acetylcholine receptors

T2 - A method for study of isolated neurons of the adult central nervous system in the turtle

AU - James, William L.

AU - Klein, William L.

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Y1 - 1982/9/20

N2 - Adult neurons were isolated from turtle retina in order to study the subcellular distribution of muscarinic acetylcholine receptors. Retinas were perfused in vitro with [3H]quinuclidinylbenzilate [3H]QNB) to label muscarinic receptors, and individual neurons with extensive dendritic arborization were obtained for autoradiography by use of papain and trituration. Papain caused no change in the number of muscarinic receptor sites nor in their affinity for [3H]QNB. Receptors, indicated by the positions of silver grains, were localized to dendritic processes.

AB - Adult neurons were isolated from turtle retina in order to study the subcellular distribution of muscarinic acetylcholine receptors. Retinas were perfused in vitro with [3H]quinuclidinylbenzilate [3H]QNB) to label muscarinic receptors, and individual neurons with extensive dendritic arborization were obtained for autoradiography by use of papain and trituration. Papain caused no change in the number of muscarinic receptor sites nor in their affinity for [3H]QNB. Receptors, indicated by the positions of silver grains, were localized to dendritic processes.

KW - autoradiography

KW - cholinergic

KW - dendrites

KW - muscarinic

KW - receptor localization

KW - retina

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Autoradiography of dendritic acetylcholine receptors: A method for study of isolated neurons of the adult central nervous system in the turtle

Abstract

Keywords

ASJC Scopus subject areas

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