Avidity enhancement of L-selectin bonds by flow: Shear-promoted rotation of leukocytes turn labile bonds into functional tethers

Oren Dwir, Ariel Solomon, Shmuel Mangan, Geoffrey S. Kansas, Ulrich S. Schwarz, Ronen Alon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

L-selectin is a key lectin essential for leukocyte capture and rolling on vessel walls. Functional adhesion of L-selectin requires a minimal threshold of hydrodynamic shear. Using high temporal resolution videomicroscopy, we now report that L-selectin engages its ligands through exceptionally labile adhesive bonds (tethers) even below this shear threshold. These tethers share a lifetime of 4 ms on distinct physiological ligands, two orders of magnitude shorter than the lifetime of the P-selectin-PSGL-1 bond. Below threshold shear, tether duration is not shortened by elevated shear stresses. However, above the shear threshold, selectin tethers undergo 14-fold stabilization by shear-driven leukocyte transport. Notably, the cytoplasmic tail of L-selectin contributes to this stabilization only above the shear threshold. These properties are not shared by P-selectin- or VLA-4-mediated tethers. L-selectin tethers appear adapted to undergo rapid avidity enhancement by cellular transport, a specialized mechanism not used by any other known adhesion receptor.

Original languageEnglish (US)
Pages (from-to)649-659
Number of pages11
JournalJournal of Cell Biology
Volume163
Issue number3
DOIs
StatePublished - Nov 10 2003

Keywords

  • Aggregation
  • Lymph nodes
  • Rolling
  • Selectin
  • Shear flow

ASJC Scopus subject areas

  • Cell Biology

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