Axon guidance of mouse olfactory sensory neurons by odorant receptors and the β2 adrenergic receptor

Paul Feinstein*, Thomas Bozza, Ivan Rodriguez, Anne Vassalli, Peter Mombaerts

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

225 Scopus citations

Abstract

Odorant receptors (ORs) provide the core determinant of identity for axons of olfactory sensory neurons (OSNs) to coalesce into glomeruli in the olfactory bulb. Here, using gene targeting in mice, we examine how the OR protein determines axonal identity. An OR::GFP fusion protein is present in axons, consistent with a direct function of ORs in axon guidance. When the OR coding region is deleted, we observe OSNs that coexpress other ORs that function in odorant reception and axonal identity. It remains unclear if such coexpression is normally prevented by negative feedback on OR gene choice. A drastic reduction in OR protein level produces axonal coalescence into novel, remote glomeruli. By contrast, chimeric ORs and ORs with minor mutations perturb axon outgrowth. Strikingly, the β2 adrenergic receptor can substitute for an OR in glomerular formation when expressed from an OR locus. Thus, ORs have not evolved a unique function in axon guidance.

Original languageEnglish (US)
Pages (from-to)833-846
Number of pages14
JournalCell
Volume117
Issue number6
DOIs
StatePublished - Jun 11 2004

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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