TY - JOUR
T1 - Bacillus subtilis genome project
T2 - cloning and sequencing of the 97 kb region from 325° to 333deg;
AU - Glaser, P.
AU - Kunst, F.
AU - Arnaud, M.
AU - Coudart, M. ‐P
AU - Gonzales, W.
AU - Hullo, M. ‐F
AU - Ionescu, M.
AU - Lubochinsicy, B.
AU - Marcelino, L.
AU - Moszer, I.
AU - Presecan, E.
AU - Santana, M.
AU - Schneider, E.
AU - Schwelzer, J.
AU - Vertes, A.
AU - Rapoport, G.
AU - Danchin, A.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety‐two open reading frames were predicted to encode putative proteins among which only forty‐two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis.
AB - In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety‐two open reading frames were predicted to encode putative proteins among which only forty‐two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=0027423563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027423563&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1993.tb01963.x
DO - 10.1111/j.1365-2958.1993.tb01963.x
M3 - Article
C2 - 7934828
AN - SCOPUS:0027423563
VL - 10
SP - 371
EP - 384
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 2
ER -