Bacteria as solid phase in a concentration fluorescence immunoassay analysis of antibodies to surface antigens

William R. Schwan*, Carl Waltenbaugh, James L. Duncan

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A modified solid-phase fluorescence immunoassay was developed using bacterial cells as the solid phase to screen antibodies produced against surface antigens from a clinical isolate of Escherichia coli, strain 1-149. The bacterial solid phase was used to analyze both polyclonal and monoclonal antibodies. The bacterial concentration fluorescence immunoassay (BCFIA) showed up to 50-fold greater sensitivity in bacterial cell detection as compared to ELISA (enzyme-linked immunosorbent assay). Moreover, BCFIA was considerably faster than ELISA with uniform reproducibility. This paper demonstrates the utility of using bacteria and their surface antigens as solid-phase matrices for antibody characterization in a FIA.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalJournal of Immunological Methods
Volume126
Issue number2
DOIs
StatePublished - Feb 9 1990

Keywords

  • Bacteria concentration fluorescence immunoassay
  • Escherichia coli
  • Monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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