Band broadening theories in capillary electrophoresis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In capillary electrophoresis (CE), analytes are separated along the axis of a single microcapillary by virtue of their differential migration in an applied electric field. CE can also be performed in channels etched on solid substrates such as glass or PDMS and can be integrated into a microfluidic chip with a complex network of electric and fluidic circuits. The measure of quality of a CE instrument is resolution which is limited fundamentally by mixing due to various physical processes. The theoretical limit on the best separation that can be achieved is set by molecular diffusion, which is inevitable. The goal is to eliminate or minimize the other sources of dispersion by design. This chapter provides an overview of the various mechanisms of band broadening and the mathematical results that make it possible to estimate their relative contributions.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages143-166
Number of pages24
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1906
ISSN (Print)1064-3745

Fingerprint

Capillary Electrophoresis
Physical Phenomena
Microfluidics
Glass

Keywords

  • Debye layer
  • Electroosmosis
  • Electrophoresis
  • Taylor dispersion
  • Zeta potential

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ghosal, S. (2019). Band broadening theories in capillary electrophoresis. In Methods in Molecular Biology (pp. 143-166). (Methods in Molecular Biology; Vol. 1906). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8964-5_11
Ghosal, Sandip. / Band broadening theories in capillary electrophoresis. Methods in Molecular Biology. Humana Press Inc, 2019. pp. 143-166 (Methods in Molecular Biology).
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Ghosal, S 2019, Band broadening theories in capillary electrophoresis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1906, Humana Press Inc, pp. 143-166. https://doi.org/10.1007/978-1-4939-8964-5_11

Band broadening theories in capillary electrophoresis. / Ghosal, Sandip.

Methods in Molecular Biology. Humana Press Inc, 2019. p. 143-166 (Methods in Molecular Biology; Vol. 1906).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Ghosal S. Band broadening theories in capillary electrophoresis. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 143-166. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8964-5_11