Barcoding Biological Reactions with DNA-Functionalized Vesicles

Justin A. Peruzzi; Miranda L. Jacobs; Timothy Q. Vu; Kenneth S. Wang; Neha P. Kamat

doi:10.1002/anie.201911544

Barcoding Biological Reactions with DNA-Functionalized Vesicles

Justin A. Peruzzi, Miranda L. Jacobs, Timothy Q. Vu, Kenneth S. Wang, Neha P. Kamat^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Targeted vesicle fusion is a promising approach to selectively control interactions between vesicle compartments and would enable the initiation of biological reactions in complex aqueous environments. Here, we explore how two features of vesicle membranes, DNA tethers and phase-segregated membranes, promote fusion between specific vesicle populations. Membrane phase-segregation provides an energetic driver for membrane fusion that increases the efficiency of DNA-mediated fusion events. The orthogonality provided by DNA tethers allows us to direct fusion and delivery of DNA cargo to specific vesicle populations. Vesicle fusion between DNA-tethered vesicles can be used to initiate in vitro protein expression to produce model soluble and membrane proteins. Engineering orthogonal fusion events between DNA-tethered vesicles provides a new strategy to control the spatiotemporal dynamics of cell-free reactions, expanding opportunities to engineer artificial cellular systems.

Original language	English (US)
Pages (from-to)	18683-18690
Number of pages	8
Journal	Angewandte Chemie - International Edition
Volume	58
Issue number	51
DOIs	https://doi.org/10.1002/anie.201911544
State	Published - Dec 16 2019

Keywords

artificial cell
biophysics
cell-free reactions
membrane fusion
vesicles

ASJC Scopus subject areas

Chemistry(all)
Catalysis

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10.1002/anie.201911544

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abstract = "Targeted vesicle fusion is a promising approach to selectively control interactions between vesicle compartments and would enable the initiation of biological reactions in complex aqueous environments. Here, we explore how two features of vesicle membranes, DNA tethers and phase-segregated membranes, promote fusion between specific vesicle populations. Membrane phase-segregation provides an energetic driver for membrane fusion that increases the efficiency of DNA-mediated fusion events. The orthogonality provided by DNA tethers allows us to direct fusion and delivery of DNA cargo to specific vesicle populations. Vesicle fusion between DNA-tethered vesicles can be used to initiate in vitro protein expression to produce model soluble and membrane proteins. Engineering orthogonal fusion events between DNA-tethered vesicles provides a new strategy to control the spatiotemporal dynamics of cell-free reactions, expanding opportunities to engineer artificial cellular systems.",

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author = "Peruzzi, {Justin A.} and Jacobs, {Miranda L.} and Vu, {Timothy Q.} and Wang, {Kenneth S.} and Kamat, {Neha P.}",

note = "Funding Information: We thank members of the Kamat Lab for thoughtful discussions. This research was supported in part by the Searle Funds at The Chicago Community Trust and the National Science Foundation under Grant No. 1844336. J.A.P. was supported by an NSF Graduate Research Fellowship. M.L.J. was supported by Grant Number T32GM008382 from the National Institute of General Medical Sciences. This work made use of the Keck Biophysics Facility and IMSERC at Northwestern University. IMSERC has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the State of Illinois, and the International Institute for Nanotechnology (IIN). Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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AU - Kamat, Neha P.

N1 - Funding Information: We thank members of the Kamat Lab for thoughtful discussions. This research was supported in part by the Searle Funds at The Chicago Community Trust and the National Science Foundation under Grant No. 1844336. J.A.P. was supported by an NSF Graduate Research Fellowship. M.L.J. was supported by Grant Number T32GM008382 from the National Institute of General Medical Sciences. This work made use of the Keck Biophysics Facility and IMSERC at Northwestern University. IMSERC has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the State of Illinois, and the International Institute for Nanotechnology (IIN). Publisher Copyright: © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2019/12/16

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Barcoding Biological Reactions with DNA-Functionalized Vesicles

Abstract

Keywords

ASJC Scopus subject areas

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