Basal cell-specific and hyperproliferation-related keratins in human breast cancer

R. H W Wetzels*, H. J H Kuijpers, E. B. Lane, I. M. Leigh, S. M. Troyanovsky, R. Holland, U. J G M Van Haelst, F. C S Ramaekers

*Corresponding author for this work

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150 Scopus citations

Abstract

In normal breast tissue and in noninvasive breast carcinomas, various keratin-14 antibodies were reactive predominantly with the basal/myoepithelial cell layer, although mainly in terminal and larger ducts luminal cells sometimes also were stained. A similar reaction pattern was found with an antibody directed against keratin 17, although this antibody was more often found negative than keratin 14 in the pre-existing myoepithelial cells in intraductal carcinomas. Furthermore antibodies reactive with hyperproliferation-related keratins 6 and 16 were used. One of these (LL025) was completely negative in normal breast tissue and noninvasive breast carcinomas. However 10% of the invasive carcinomas were diffusely or focally positive with this latter antibody, while in 18 of 115 cases of invasive breast carcinomas studied, a basal cell phenotype was detected. A relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.

Original languageEnglish (US)
Pages (from-to)751-763
Number of pages13
JournalAmerican Journal of Pathology
Volume138
Issue number3
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Wetzels, R. H. W., Kuijpers, H. J. H., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., Van Haelst, U. J. G. M., & Ramaekers, F. C. S. (1991). Basal cell-specific and hyperproliferation-related keratins in human breast cancer. American Journal of Pathology, 138(3), 751-763.