Basophils produce IL-4 and accumulate in tissues after infection with a Th2-inducing parasite

Booki Min, Melanie Prout, Jane Hu-Li, Jinfang Zhu, Dragana Jankovic, Ellen S. Morgan, Joseph F. Urban, Ann M. Dvorak, Fred D. Finkelman, Graham LeGros, William E. Paul*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

336 Scopus citations

Abstract

Using mice in which the eGfp gene replaced the first exon of the Il4 gene (G4 mice), we examined production of interleukin (IL)-4 during infection by the intestinal nematode Nippostrongylus brasiliensis (Nb). Nb infection induced green fluorescent protein (GFP)pos cells that were FcεRI pos CD49bbright, c-kitneg and Gr1 neg. These cells had lobulated nuclei and granules characteristic of basophils. They were found mainly in the liver and lung, to a lesser degree in the spleen, but not in the lymph nodes. Although some liver basophils from naive mice express GFP, Nb infection enhanced GFP expression and increased the number of tissue basophils. Similar basophil GFP expression was found in infected Stat6-/- mice. Basophils did not increase in number in infected Rag2-/- mice; Rag2-/- mice reconstituted with CD4 T cells allowed significant basophil accumulation, indicating that CD4 T cells can direct both tissue migration of basophils and enhanced IL-4 production. IL-4 production was immunoglobulin independent and only partially dependent on IL-3. Thus, infection with a parasite that induces a "Th2-type response" resulted in accumulation of tissue basophils, and these cells, stimulated by a non-FcR cross-linking mechanism, are a principal source of in vivo IL-4 production.

Original languageEnglish (US)
Pages (from-to)507-517
Number of pages11
JournalJournal of Experimental Medicine
Volume200
Issue number4
DOIs
StatePublished - Aug 16 2004
Externally publishedYes

Keywords

  • CD4 T cells
  • Cytokine
  • Green fluorescent protein
  • Liver
  • Nippostrongylus brasiliensis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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