Bcs1p, an AAA-family member, is a chaperone for the assembly of the cytochrome bc1 complex

Cristina Maria Cruciat, Kai Hell, Heike Fölsch, Walter Neupert, Rosemary A. Stuart*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Bcs1p, a mitochondrial protein and member of the conserved AAA protein family, is involved in the biogenesis of the cytochrome bc1 complex. We demonstrate here that Bcs1p is directly required for the assembly of the Rieske FeS and Qcr10p proteins into the cytochrome bc1 complex. Bcs1p binds to a precomplex in the assembly pathway of the cytochrome bc1 complex. Binding of Bcs1p to and release from this assembly intermediate is driven by ATP hydrolysis. We propose that Bcs1p acts as an ATP-dependent chaperone, maintaining the precomplex in a competent state for the subsequent assembly of the Rieske FeS and Qcr10p proteins.

Original languageEnglish (US)
Pages (from-to)5226-5233
Number of pages8
JournalEMBO Journal
Volume18
Issue number19
DOIs
StatePublished - Oct 1 1999

Keywords

  • AAA family
  • Bcs1p
  • Chaperones
  • Cytochrome bc complex
  • Mitochondria

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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