@article{055606179f4f484b9a7353f6d1c758ee,
title = "Best practices and benchmarks for intact protein analysis for top-down mass spectrometry",
abstract = "One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.",
author = "Donnelly, {Daniel P.} and Rawlins, {Catherine M.} and DeHart, {Caroline J.} and Luca Fornelli and Schachner, {Luis F.} and Ziqing Lin and Lippens, {Jennifer L.} and Aluri, {Krishna C.} and Richa Sarin and Bifan Chen and Carter Lantz and Wonhyeuk Jung and Johnson, {Kendall R.} and Antonius Koller and Wolff, {Jeremy J.} and Campuzano, {Iain D.G.} and Auclair, {Jared R.} and Ivanov, {Alexander R.} and Whitelegge, {Julian P.} and Ljiljana Pa{\v s}a-Toli{\'c} and Julia Chamot-Rooke and Danis, {Paul O.} and Smith, {Lloyd M.} and Tsybin, {Yury O.} and Loo, {Joseph A.} and Ying Ge and Kelleher, {Neil L.} and Agar, {Jeffrey N.}",
note = "Funding Information: J.N.A. was supported in part by NIH R01 NS065263 and ALSA 18-IIA-420 in collaboration with Biopharmaceutical Analysis Training Lab at Northeastern University. Y.G. was supported by NIH R01 GM117058 and S10 OD018475 and acknowledges the UW-Madison Human Proteomics Program Mass Spectrometry Facility (initially funded by the Wisconsin Partnership Funds). A.R.I. was supported by the NIH awards 1R01GM120272 and R01CA218500 and acknowledges Thermo Fisher Scientific and SCIEX for their support. J.P.W.{\textquoteright}s lab was supported in part by the NIH grant P30DK063491. J.A.L. acknowledges the NIH (R01 GM103479, S10 RR028893, S10 OD018504) and the US Department of Energy (DE-FC-02-02ER63421). J.L.L. acknowledges the postdoctoral program at Amgen, Inc. C.L. acknowledges support from the NIH Ruth L. Kirschstein National Research Service Award (NRSA; T32 GM007185). Work done by N.L.K. was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under NIH Grant P41GM108569 from the National Institute of General Medical Sciences. The NRTDP would like to thank I. Ntai for assistance in data collection. L. Schachner is a Howard Hughes Medical Institute Gilliam Fellow. Research reported in this publication was supported by a fellowship associated with the Chemistry of Life Processes Predoctoral Training Program T32GM105538 at Northwestern University. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2019",
month = jul,
day = "1",
doi = "10.1038/s41592-019-0457-0",
language = "English (US)",
volume = "16",
pages = "587--594",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "7",
}