Best practices for managing large CryoEM facilities

Bart Alewijnse; Alun W. Ashton; Melissa G. Chambers; Songye Chen; Anchi Cheng; Mark Ebrahim; Edward T. Eng; Wim J.H. Hagen; Abraham J. Koster; Claudia S. López; Natalya Lukoyanova; Joaquin Ortega; Ludovic Renault; Steve Reyntjens; William J. Rice; Giovanna Scapin; Raymond Schrijver; Alistair Siebert; Scott M. Stagg; Valerie Grum-Tokars; Elizabeth R. Wright; Shenping Wu; Zhiheng Yu; Z. Hong Zhou; Bridget Carragher; Clinton S. Potter

doi:10.1016/j.jsb.2017.07.011

Best practices for managing large CryoEM facilities

Bart Alewijnse, Alun W. Ashton, Melissa G. Chambers, Songye Chen, Anchi Cheng, Mark Ebrahim, Edward T. Eng, Wim J.H. Hagen, Abraham J. Koster, Claudia S. López, Natalya Lukoyanova, Joaquin Ortega, Ludovic Renault, Steve Reyntjens, William J. Rice, Giovanna Scapin, Raymond Schrijver, Alistair Siebert, Scott M. Stagg, Valerie Grum-TokarsElizabeth R. Wright, Shenping Wu, Zhiheng Yu, Z. Hong Zhou, Bridget Carragher, Clinton S. Potter^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Review article › peer-review

28 Scopus citations

Abstract

This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

Original language	English (US)
Pages (from-to)	225-236
Number of pages	12
Journal	Journal of Structural Biology
Volume	199
Issue number	3
DOIs	https://doi.org/10.1016/j.jsb.2017.07.011
State	Published - Sep 1 2017

Funding

The workshop was part of the activities of the National Resource for Automated Molecular Microscopy at the Simons Electron Microscopy Facility that is supported by grants from NIH NIGMS GM103310 and the Simons Foundation ( 349247 ). Additional financial support for travel and other costs directly related to the workshop was provided by FEI Co./Thermo Fisher Scientific . Appendix A

Keywords

Automation
Cryotem
Data management
Facility management
Single-particle
User training

ASJC Scopus subject areas

Structural Biology

Access to Document

10.1016/j.jsb.2017.07.011

Cite this

Alewijnse, B., Ashton, A. W., Chambers, M. G., Chen, S., Cheng, A., Ebrahim, M., Eng, E. T., Hagen, W. J. H., Koster, A. J., López, C. S., Lukoyanova, N., Ortega, J., Renault, L., Reyntjens, S., Rice, W. J., Scapin, G., Schrijver, R., Siebert, A., Stagg, S. M., ... Potter, C. S. (2017). Best practices for managing large CryoEM facilities. Journal of Structural Biology, 199(3), 225-236. https://doi.org/10.1016/j.jsb.2017.07.011

@article{93a1d169081c40b4962b70a76434fc9d,

title = "Best practices for managing large CryoEM facilities",

abstract = "This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.",

keywords = "Automation, Cryotem, Data management, Facility management, Single-particle, User training",

author = "Bart Alewijnse and Ashton, {Alun W.} and Chambers, {Melissa G.} and Songye Chen and Anchi Cheng and Mark Ebrahim and Eng, {Edward T.} and Hagen, {Wim J.H.} and Koster, {Abraham J.} and L{\'o}pez, {Claudia S.} and Natalya Lukoyanova and Joaquin Ortega and Ludovic Renault and Steve Reyntjens and Rice, {William J.} and Giovanna Scapin and Raymond Schrijver and Alistair Siebert and Stagg, {Scott M.} and Valerie Grum-Tokars and Wright, {Elizabeth R.} and Shenping Wu and Zhiheng Yu and Zhou, {Z. Hong} and Bridget Carragher and Potter, {Clinton S.}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = sep,

day = "1",

doi = "10.1016/j.jsb.2017.07.011",

language = "English (US)",

volume = "199",

pages = "225--236",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press Inc.",

number = "3",

}

Alewijnse, B, Ashton, AW, Chambers, MG, Chen, S, Cheng, A, Ebrahim, M, Eng, ET, Hagen, WJH, Koster, AJ, López, CS, Lukoyanova, N, Ortega, J, Renault, L, Reyntjens, S, Rice, WJ, Scapin, G, Schrijver, R, Siebert, A, Stagg, SM, Grum-Tokars, V, Wright, ER, Wu, S, Yu, Z, Zhou, ZH, Carragher, B & Potter, CS 2017, 'Best practices for managing large CryoEM facilities', Journal of Structural Biology, vol. 199, no. 3, pp. 225-236. https://doi.org/10.1016/j.jsb.2017.07.011

TY - JOUR

T1 - Best practices for managing large CryoEM facilities

AU - Alewijnse, Bart

AU - Ashton, Alun W.

AU - Chambers, Melissa G.

AU - Chen, Songye

AU - Cheng, Anchi

AU - Ebrahim, Mark

AU - Eng, Edward T.

AU - Hagen, Wim J.H.

AU - Koster, Abraham J.

AU - López, Claudia S.

AU - Lukoyanova, Natalya

AU - Ortega, Joaquin

AU - Renault, Ludovic

AU - Reyntjens, Steve

AU - Rice, William J.

AU - Scapin, Giovanna

AU - Schrijver, Raymond

AU - Siebert, Alistair

AU - Stagg, Scott M.

AU - Grum-Tokars, Valerie

AU - Wright, Elizabeth R.

AU - Wu, Shenping

AU - Yu, Zhiheng

AU - Zhou, Z. Hong

AU - Carragher, Bridget

AU - Potter, Clinton S.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

AB - This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

KW - Automation

KW - Cryotem

KW - Data management

KW - Facility management

KW - Single-particle

KW - User training

UR - http://www.scopus.com/inward/record.url?scp=85028364106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028364106&partnerID=8YFLogxK

U2 - 10.1016/j.jsb.2017.07.011

DO - 10.1016/j.jsb.2017.07.011

M3 - Review article

C2 - 28827185

AN - SCOPUS:85028364106

SN - 1047-8477

VL - 199

SP - 225

EP - 236

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 3

ER -

Best practices for managing large CryoEM facilities

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this